Research progress on recombinase polymerase amplification

Abstract:

Recombinase polymerase amplification (RPA) is a technology that utilizes proteins such as recombinase (T4 UvsX), single-stranded DNA binding protein (T4 Gp32), and strand replacement DNA polymerase (Bsu) to amplify target nucleic acids at constant temperatures. In recent years, RPA plays a unique role in the detection of bacteria, viruses, parasites, mycoplasma, and chlamydia for it can amplify the target nucleic acid fragment with constant temperature and high efficiency, it gradually becomes a key technology for point-of-care testing (POCT). This review takes the principle of RPA technology as a starting point, it describes the clinical application, shortcomings, and improvement of RPA, and RPA is expected to provide a new idea for the rapid detection and diagnosis of clinical diseases.