Isolation, identification, and probiotic properties of lactic acid bacteria with tolerance to slightly acidic hypochlorous acid water

Abstract:

[Background] The bactericidal hypochlorous acid water may negatively affect the beneficial bacteria in the intestinal tracts of domesticated animals when being used as drinking water. [Objective] To isolate the lactic acid bacteria with tolerance to slightly acidic hypochlorous acid water (SAHW) and investigate their probiotic properties. [Methods] Eight strains of Lactobacillus were isolated from the traditional natural fermentation products in Inner Mongolia and identified by 16S rRNA gene sequencing. The tolerance to SAHW, acid, and bile salt, hydrophobicity, and self-agglutination properties of the isolates were examined. [Results] The strain MBH3-2 was identified as Lactobacillus paracasei. After treatment with 20 mg/L SAHW for 2 h, the strain showed the count decrease of 0.3 lg(CFU/mL) and the survival rate of 50.10%. MBH3-2 was highly hydrophobic with the hydrophobicity of 62.12%, and its self-agglutination rate reached 97.29% at the time point of 2 h. [Conclusion] Strain MBH3-2 with strong tolerance to SAHW and basically meeting the requirements as a microecological preparation was identified, demonstrating the potential to be developed as a new microecological preparation.