Research status and prospect of the synthesis of friedelin and its derivatives by Saccharomyces cerevisiae

Abstract:

Friedelin and its derivatives are ubiquitous in plants, with a wide variety of physiological and pharmacological activities. The derivatives of friedelin are modified from friedelin by cytochrome P450 oxidase (CYP450) and UDP-glucuronosyltransferase (UGT). Because of the extremely low content of natural friedelin and its derivatives in plants, the traditional extraction and chemical synthesis methods are inefficient, energy-consuming, and pollute the environment. Therefore, using Saccharomyces cerevisiae as the host bacteria to produce friedelin and its derivatives is an efficient and environmentally friendly approach. In this paper, we introduced and looked forward to the efficient production of friedelin in S. cerevisiae from the aspects of increasing precursor content, improving enzyme activity, and subcellular localization of product synthesis. In addition, this paper reviewed the current research status of several common friedelin derivatives, and put forward new ways for the synthesis of friedelin derivatives from the aspects of mining CYP450 based on carbon skeleton similarity, modifying CYP450 by protein engineering, and mining the gene clusters involved in the synthesis.

Key words:Saccharomyces cerevisiae;pentacyclic triterpene of friedelanes;metabolic pathway;synthetic biology