Active components and main pathways involved in fruiting body development of Lanmaoa asiatica

Abstract:

[Background] The fruiting body formation and development of ectomycorrhizal fungi such as Lanmaoa asiatica still unknown. [Objective] To identify the active components regulating the fruiting body development. [Methods] We employed Three metabolomics techniques including nuclear magnetic resonance, gas chromatography-mass spectrometry, and liquid chromatography-mass spectrometry were employed to analyze the small molecular components in L. asiatica primordia cultured for 8 days (Y8) and feral sterile fruiting bodies (Z0). [Results] We found that each of the three analytical methods identified 451 and 473 compounds in Y8 and Z0, respectively. A total of 362 significantly differential components were identified, including 206 up-regulated components and 156 down-regulated components, which were involved in 47 pathways. [Conclusion] We hypothesized that the deep transformation and regulation of components were accomplished via nine main pathways. The significantly up-regulated and down-regulated differential components such as bolegrevilol may play a regulatory role in the development of fruiting bodies. The three methods used in this study complemented each other, expanding the scope and improving the sensitivity of the detection. The results provided some theoretical reference for deciphering the mechanism of fruiting body development and exploring the artificial cultivation of L. asiatica.