Effects of five mineral elements on mycelial growth and active oxygen scavenging ability of Volvariella volvacea

Abstract:

[Background] Strain degradation is a difficult problem in the production of Volvariella volvacea. Exploring a simple and efficient rejuvenation method is an urgent problem to be solved in the V. volvacea industry. [Objective] To investigate the effects of exogenous addition of five mineral elements on mycelial traits and active oxygen scavenging ability of degraded strains of V. volvacea. [Methods] The optimum concentrations of five mineral elements were selected and added to the potato dextrose agar (PDA) medium. The colony morphology, mycelial growth rate, mycelial biomass, active oxygen content, antioxidant enzyme activity, and antioxidant substance content of V. volvacea were determined. [Results] MnSO₄, Na₂SeO₃, CaSO₄, and FeSO₄ could effectively improve the mycelial growth rate and biomass of the degraded strains D1 and D2. The content of active oxygens such as O₂·^- and H₂O₂ was decreased, and the content of reduced glutathione (GSH) and oxidized glutathione (GSSG) and the activity of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), and glutathione reductase (GR) were increased. However, ZnSO₄ had no significant effect on mycelial traits and active oxygen scavenging ability of D1 and D2. [Conclusion] Exogenous addition of MnSO₄, Na₂SeO₃, CaSO₄, and FeSO₄ can effectively improve the mycelial growth and active oxygen scavenging ability of V. volvacea, and the effect of MnSO₄ was optimal.

Key words:Volvariella volvacea;mineral elements;mycelial traits;active oxygen species;antioxidant enzymes;strain rejuvenation