Isolation, identification, and antimicrobial activities of myxobacteria in primitive forest of Tianshan Grand Canyon

doi:10.13344/j.microbiol.china.230009

Home > Archive>Volume 50, Issue 9, 2023 >3952-3969. DOI:10.13344/j.microbiol.china.230009

Isolation, identification, and antimicrobial activities of myxobacteria in primitive forest of Tianshan Grand Canyon
DOI:
                        10.13344/j.microbiol.china.230009
                    
CSTR:
                        32113.14.j.MC.230009
                    
Author:
                        DOU XinyuDOU Xinyu
Key Laboratory of the Pest Monitoring and Safety Control of Crops and Forests of Xinjiang Uygur Autonomous Region, Key Laboratory of Prevention and Control of Invasive Alien Species in Agriculture & Forestry of the North-western Desert Oasis(Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, Xinjiang, China
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PAN WenPAN Wen
Key Laboratory of the Pest Monitoring and Safety Control of Crops and Forests of Xinjiang Uygur Autonomous Region, Key Laboratory of Prevention and Control of Invasive Alien Species in Agriculture & Forestry of the North-western Desert Oasis(Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, Xinjiang, China
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DONG ZhimingDONG Zhiming
Key Laboratory of the Pest Monitoring and Safety Control of Crops and Forests of Xinjiang Uygur Autonomous Region, Key Laboratory of Prevention and Control of Invasive Alien Species in Agriculture & Forestry of the North-western Desert Oasis(Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, Xinjiang, China
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LUO MingLUO Ming
Key Laboratory of the Pest Monitoring and Safety Control of Crops and Forests of Xinjiang Uygur Autonomous Region, Key Laboratory of Prevention and Control of Invasive Alien Species in Agriculture & Forestry of the North-western Desert Oasis(Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, Xinjiang, China
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HAN JianHAN Jian
Key Laboratory of the Pest Monitoring and Safety Control of Crops and Forests of Xinjiang Uygur Autonomous Region, Key Laboratory of Prevention and Control of Invasive Alien Species in Agriculture & Forestry of the North-western Desert Oasis(Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, Xinjiang, China
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[Background] Myxobacteria are a group of higher prokaryotes with social behavior. They are versatile predators that prey on phytopathogenic fungi and bacteria, serving as excellent candidates for biocontrol agents. [Objective] To explore the diversity and antimicrobial activities of culturable myxobacteria in the primitive forest of Tianshan Grand Canyon in Urumqi, and lay a foundation for the discovery of myxobacteria strains for biocontrol. [Methods] Culturable myxobacteria were isolated from the soil and rotting wood samples collected from the primitive forest of Tianshan Grand Canyon by the rabbit dung pellets-inducing method and prey-inducing method. The isolates were identified based morphological characteristics, physiological and biochemical properties, and the 16S rRNA gene sequences. The antimicrobial activities of the isolates were determined with 6 phytopathogenic fungal species (Verticillium dahliae, Fusarium oxysporum f. sp. vasinfectum, Fusarium verticillioides, Rhizoctonia solani, Fusarium culmorum, and Alternaria tenuissima) and 1 phytopathogenic bacterial species (Erwinia amylovora) by plate confrontation method and lawn predation method. [Results] Seventy potential strains were isolated from the collected samples, and 36 pure cultures of myxobacteria were obtained after purification. The 36 strains belonged to 4 genera: Myxococcus (30 strains), Cystobacter (3 strains), Corallococcus (2 strains), and Archangium (1 strain). The 36 strains of myxobacteria presented activities against at least two species of phytopathogenic fungi, exhibiting broad-spectrum antifungal activities. One strain NSE37-1 with both broad-spectrum efficient antifungal activity was screened out. Fifteen strains had predatory activity against E. amylovora, and one strain NSE25 with strong predatory activity was screened out. [Conclusion] The culturable myxobacteria are abundant in Tianshan Grand Canyon, among which Myxococcus is the dominant genus. The isolated and purified myxobacteria strains all exhibited broad-spectrum activities against phytopathogens, demonstrating the value for research in the future.

Key words:Tianshan Grand Canyon;primitive forest;myxobacteria;isolation and identification;antimicrobial activity

Reference

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DOU Xinyu, PAN Wen, DONG Zhiming, LUO Ming, HAN Jian. Isolation, identification, and antimicrobial activities of myxobacteria in primitive forest of Tianshan Grand Canyon[J]. Microbiology China, 2023, 50(9): 3952-3969

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Received:January 04,2023
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Online: September 04,2023
Published: September 20,2023

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