Screening and growth characteristics of an albite-decomposing strain

Abstract:

[Background] The sodium in albite poses a challenge to mineral processing and utilization. [Objective] To screen an efficient albite-decomposing strain and to remove the sodium in albite. [Methods] With albite as the sole sodium source, a stable strain with strong decomposition ability was screened from the soil of salt lake in Inner Mongolia. Then, it was identified based on morphological observation, physiological and biochemical characteristics, and 16S rRNA gene sequencing. The phylogenetic tree was constructed and the growth curve was plotted. Moreover, the influence of culture conditions of the growth of the strain was investigated. [Results] An efficient albite-decomposing strain was screened out and identified as Acinetobacter johnsonⅡ, with the logarithmic phase of the 3rd−11th hours. The optimal culture conditions are as follows:initial pH 9.0, temperature of 25℃, 2% salt, shaker speed at 150 r/min, inoculum of 5%, liquid volume of 100 mL/250 mL, beef extract as the sole carbon source, and peptone as the sole nitrogen source. [Conclusion] It is reported for the first time that A. johnsonⅡ has the ability to decompose albite, which provides a strain for biological removal of sodium.

Key words:albite;screening;Acinetobacter johnsonⅡ;growth characteristics;biological removal of sodium