Screening of tris-(1-chloro-2-propyl) phosphate-degrading bacteria and its degradation characteristics

Abstract:

[Background] Tris-(1-chloro-2-propyl) phosphate (TCIPP), as an emerging organic pollutant of global wide concern, has the characteristics of high environmental concentration, difficult biodegradation, etc. It is urgent to develop efficient removal technology for TCIPP. [Objective] To obtain a new strain with high TCIPP degradation efficiency that can be used for TCIPP pollution remediation.[Methods] The strain which could degrade TCIPP with high concentration (up to 100 mg/L) in liquid was isolated from the TCIPP-contaminated soil by gradually increasing the concentration of TCIPP in the mineral salt medium. The strain was preliminarily identified according to 16S rRNA gene sequence analysis, and its characteristics of degrading TCIPP in liquid were further investigated. [Results] The strain DT-6 was identified as Ochrobactrum sp., and it could utilize TCIPP as the only carbon and energy source. When the initial concentration of TCIPP was 50 mg/L and the incubation time was 7 d, the biomass of the DT-6 strain was the largest and the degradation rate of TCIPP reached the highest (34.6%). The addition of sucrose significantly promoted the growth of the DT-6 strain but inhibited the degradation of TCIPP. [Conclusion] This study reported a highly efficient TCIPP-degrading strain, Ochrobactrum sp. DT-6, which could provide new germplasm resources for the bioremediation of TCIPP pollution in the environment.