Seasonal concentrations and influencing factors of airborne microbes in Lhasa of China

doi:10.13344/j.microbiol.china.220601

Home > Archive>Volume 50, Issue 3, 2023 >954-968. DOI:10.13344/j.microbiol.china.220601

Seasonal concentrations and influencing factors of airborne microbes in Lhasa of China
DOI:
                        10.13344/j.microbiol.china.220601
                    
CSTR:
                        32113.14.j.MC.220601
                    
Author:
                        WU WenjieWU Wenjie
State Key Laboratory of Tibetan Plateau Earth System, Environment and Resources (TPESER), Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, China;University of Chinese Academy of Sciences, Beijing 100049, China
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MAO GuannanMAO Guannan
State Key Laboratory of Tibetan Plateau Earth System, Environment and Resources (TPESER), Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, China
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QI JingQI Jing
Center for the Pan-third Pole Environment, Lanzhou University, Lanzhou 730000, Gansu, China;College of Ecology, Lanzhou University, Lanzhou 730000, Gansu, China
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LIU YongqinLIU Yongqin
State Key Laboratory of Tibetan Plateau Earth System, Environment and Resources (TPESER), Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, China;Center for the Pan-third Pole Environment, Lanzhou University, Lanzhou 730000, Gansu, China;College of Ecology, Lanzhou University, Lanzhou 730000, Gansu, China
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Abstract:

[Background] Airborne microbes are a key component of the urban ecosystem, the concentration of which has great significance for monitoring urban air quality, controlling environmental pollution, and preventing disease. [Objective] To analyze the distribution characteristics of airborne microbes in Lhasa of China and explore the effects of meteorological factors and air particulate matter on the distribution of airborne microbes. [Methods] The airborne microbes were stained by SYBR Green I and observed under an epifluorescence microscope. The seasonal concentrations of the microbes from October 2019 to October 2020 were determined. Furthermore, the correlations of the concentration of airborne microbes with meteorological factors and environmental indicators were studied. [Results] The airborne microbes appeared bright green, oval, with a diameter of 0.5-1.0 μm and attaching to the organic matter and black carbon. The concentrations of total microbes in Lhasa ranged from 3.10×10³ to 2.38×10⁴ cells/m³. The concentrations of airborne microbes in two forms (free-floating and particle-attached) were the highest in winter. The concentration of free-floating microbes was the lowest in autumn, which was significantly different from that in winter (P<0.05). The concentration of particle-attached microbes showed no significant difference among four seasons. Furthermore, the concentration of airborne microbes was not significantly correlated with meteorological factors, while it had a significant positive correlation with the concentration of air particulate matter (P<0.05). [Conclusion] The concentration of airborne microbes in Lhasa was at a low level compared with that in other cities and affected by air pollutants.

Key words:airborne microbe;filter membrane sampling;fluorescence microscopy;concentration characteristics;influencing factors

Reference

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WU Wenjie, MAO Guannan, QI Jing, LIU Yongqin. Seasonal concentrations and influencing factors of airborne microbes in Lhasa of China[J]. Microbiology China, 2023, 50(3): 954-968

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Received:June 24,2022
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Online: March 07,2023
Published: March 20,2023

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