Screening of purine nucleoside-degrading lactic acid bacteria and exploration of its probiotic properties

Abstract:

[Background] Hyperuricemia is a chronic metabolic disease caused by the disorder of purine metabolism and lactic acid bacteria can degrade purines. [Objective] To screen lactic acid bacteria that efficiently degrade purine nucleoside and analyze the probiotic properties. [Methods] High performance liquid chromatography was used to determine the strain with the strongest ability to degrade inosine and guanosine. What’s more, probiotic properties of the target strain were studied by drug sensitivity test, in vitro tolerance tests, and cell adhesion test. [Results] Lactobacillus fermentum SR2-6 was screened out, which had the highest degradation rates of inosine and guanosine (99.26% and 98.85%, respectively). This strain showed no resistance to five common antibiotics such as penicillin and chloramphenicol. The survival rate of the strain was 76.51% after treatment under pH 2.0 for 4 h. The count of the viable bacteria was up to 6.85 lg (CFU/mL) after 4 h treatment in artificial satiety intestinal juice. The number bacteria adhered to Caco-2 cells was (52.29±15.14) CFU/cell. [Conclusion] SR2-6 can efficiently degrade inosine and guanosine, with excellent probiotic properties. It is a potential superior strain for the prevention and adjuvant treatment of hyperuricemia and can be applied into the development of related functional products.