Home > Archive>Volume 50, Issue 1, 2023 >1-12. DOI:10.13344/j.microbiol.china.220480
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Bioinformatics and expression analyses of glycoside hydrolase 3 genes in Trichoderma asperellum
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    Abstract:

    [Background] The glycoside hydrolase 3 gene (GH3) family mainly encodes extracellular β-glucosidases, which are the key enzymes in cellulose degradation. [Objective] To identify the GH3 genes in Trichoderma asperellum (TaGH3 genes) and investigate their transcriptional patterns. [Methods] The bioinformatics tools were employed to identify the TaGH3 genes and analyze the gene structure, phylogenetic relationship, as well as the physicochemical properties, subcellular localization, and tertiary structure of the deduced proteins. quantitative real-time polymerase chain reaction (RT-qPCR) was employed to investigate the transcriptional patterns of TaGH3 genes in the presence of cellulose. [Results] A total of 16 TaGH3 genes were identified, which contained 1-8 exons. The deduced TaGH3 proteins had the length of 533-934 amino acid (aa) and the theoretical molecular weights of 57.82-101.91 kDa. The majority of TaGH3 proteins were predicted to be extracellular. Phylogenetic analysis demonstrated that all the TaGH3 proteins could be classified into 4 clades, which had higher homology with Trichoderma reesei. Cellulose affected the transcription of all the 16 TaGH3 genes, while the transcription level varied among genes. Specifically, cellulose induced the constitutive expression of 1 gene, down-regulated the expression of 2 genes, and up-regulated the expression of 13 genes. Moreover, cellulose enhanced the extracellular β-glucosidase activity, which was consistent to the transcriptional pattern of most TaGH3 genes. [Conclusion] The genome of T. asperellum carried 16 GH3 genes, most of which encoded hydrophilic proteins with the potential for commercial exploitation. The transcription of the majority of TaGH3 genes was up-regulated by cellulose, which was consistent with the enhanced β-glucosidase activity, indicating that these genes played a key role in cellulose degradation. This study systematically investigated the GH3 genes in T. asperellum, providing new insights for the resource utilization of lignocellulosic materials and the construction of strains with high cellulase production.

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LI Cong, WANG Yun. Bioinformatics and expression analyses of glycoside hydrolase 3 genes in Trichoderma asperellum[J]. Microbiology China, 2023, 50(1): 1-12

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  • Received:May 12,2022
  • Revised:June 29,2022
  • Online: January 03,2023
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