Effect of miR-107 on expression of glycogen synthase kinase-3β and β-catenin in CVB3-infected cells

Abstract:

[Background] Abnormal expression of miR-107 can change the expression of the main proteins of Wnt/β-catenin signaling pathway in tumor cells, while miR-107 plays the same role in human cervical cancer cells (HeLa cells) infected with coxsackievirus B3 (CVB3) has not been reported. [Objective] To investigate whether miR-107 can affect the expression levels of glycogen synthase kinase-3β (GSK-3β), P-GSK-3β, and β-catenin in CVB3-infected HeLa cells. [Methods] The HeLa cells were cultured in vitro and infected with CVB3 for different time periods. The morphological changes of HeLa cells were observed by microscopy. The expression of miR-107 in HeLa cells was measured by real-time fluorescent quantitative PCR. The protein levels of GSK-3β, P-GSK-3β, β-catenin and viral capsid protein (VP1) in HeLa cells were determined by western blotting. [Results] After CVB3 infection for 6 h, the cytopathic effect was obvious. The expression level of miR-107 and the protein levels of GSK-3β, P-GSK-3β, and VP1 increased, while the protein level of β-catenin decreased, with the prolongation of CVB3 infection time (0−8 h). MiR-107 overexpression in the HeLa cells infected with CVB3 for 6 h increased the cell deaths, up-regulated the protein levels of VP1, GSK-3β, and P-GSK-3β (P<0.05), and down-regulated the protein level of β-catenin (P<0.001). The inhibition of miR-107 expression in the HeLa cells infected with CVB3 for 6 h reduced the cell deaths, down-regulated the protein levels of VP1, GSK-3β, and P-GSK-3β (P<0.05), and up-regulated the protein level of β-catenin (P<0.05). [Conclusion] Abnormal expression of miR-107 can affect the expression levels of proteins in the Wnt/β-catenin signaling pathway and VP1 in CVB3-infected HeLa cells.