Sampling strategy influences the detection results of the diversity of soil nematode-trapping fungi species

Abstract:

[Background] Soil microbial diversity has become the research frontier and hot spot in soil science, and science-based soil sampling is the prerequisite for accurate research result. [Objective] To explore the influence of sampling strategy on the detection result of soil nematode-trapping fungi (NTF) and to seek for scientific soil sampling method.[Methods] Soils were collected from different number of sampling sites (3, 5, 9 and 13) which were indifferent arrangements (straight line, V-shaped, W-shaped, triangle-like, and plum blossom-like) in 3 habitats and they were either mixed or unmixed for detection. NTF were purified by single spore isolation. The species were identified based on morphological observation and molecular techniques, and the difference in species diversity and species number of NTF among soils collected with different methods was analyzed. [Results] In the case of single-site sampling, separate purification, and integrated analysis, the α diversity and the number of detected strains rose with the increase in the integrated sampling sites. The difference in the integrated data of 5 and 9 sites was small and the detection results were relatively stable. In the instance of mixing of soil from different sites and then purification, the mixing of soil from more than 5 sites was not conducive to the detection of NTF. [Conclusion] Soil sampling strategy affects pure culture results of NTF. In the case of single-site sampling, separate purification, and integrated data analysis, the 5-site or 9-site sampling is optimal. As for the strategy of soil mixing first and then purification, the 5-site sampling is relatively scientific and feasible.

Key words:sampling strategy;pure culture;nematode-trapping fungi;alpha diversity