Research progress in rapid detection of microbial count based on ATP bioluminescence

Abstract:

The rapid detection of microbial count has always been an issue to be solved in industrial production and food industry. Adenosine triphosphate (ATP) bioluminescence method with simple operation and short detection period can meet the needs of general microbial detection. However, this method still has some limitations, such as the high limit of detection, the influence of microorganisms and other factors (non-microbial ATP, extractants, luciferase activity, etc.), all of which impact the detection results. This paper first introduced the common methods for detecting the microbial count and reviewed the development history and principle of ATP bioluminescence; expounded the effects of non-microbial ATP, free ATP, microbial biomass, ATP extractants, and luciferase on the sensitivity and stability of ATP bioluminescence method. Furthermore, summarized the application status of this method in food industry, medical services, and sewage treatment. Finally, we discussed the optimization of the detection system and the application of ATP online detection. Through this review, we aim to provide a new idea for the efficient application of ATP bioluminescence method.