Research progress of the fungal N-glycosylation system in heterologous expression

Abstract:

Increasing fungi have been developed as hosts of heterologous expressionfor the production of pharmaceutical proteins and enzymes in the last two decades. With the deepening research on the heterologous expression systems of fungi, people have gradually understood that the fungal N-glycosylation system is visibly different from that of higher animals, which has become a technological obstacle for fungi to produce higher animal-derived glycoproteins. This paper reviews the research progress of fungal N-glycosylation system in heterologous expression. Specifically, we introduced the detection techniques and transformation strategies of N-glycosylation in fungi, and compared the N-glycosylation system between fungi and higher animals, aiming to provide reference for the animalization and even humanization of fungal N-glycosylation system in the future.