Genome sequencing and mycoparasitism mechanism of a Cladosporium sp. strain

Abstract:

[Background] Cladosporium sp. SYC63 is a potential biocontrol strain with mycoparasitism and antimicrobial activity. Little is known about the whole genome sequence of this strain, which limits its development and utilization. Genome sequencing and analysis will help us understand the mycoparasitism mechanism of this strain. [Objective] The aim is to analyze the genome sequence of SYC63 and preliminarily explore the mechanism of its mycoparasitism. [Methods] The whole genome of SYC63 was sequenced on the high-throughput sequencing platform, and the genomics tools were employed for sequence assembly, gene prediction and functional annotation, prediction of secondary metabolite synthesis gene clusters, and statistical analysis of carbohydrate-active enzyme genes related to mycoparasitism. [Results] A total of 17 contigs were obtained after genome assembly, with the total length of 31 912 211 bp, the GC content of 52.80%, and 12 327 coding genes. Among them, 4 029, 949 and 6 595 genes were annotated in KEGG, COG and GO databases, respectively. At the same time, 25 gene clusters for secondary metabolite synthesis were predicted. The strain SYC63 had more glycoside hydrolase and glycolipase genes than other mycoparasitic strains (Pestalotiopsis sp., Trichoderma sp. and Coniothyrium minitans). After treatment of the spore wall with rust fungus, the expression of cell wall-degrading enzyme genes was significantly up-regulated, which revealed that the mycoparasitism mechanism of SYC63 was different from that of Trichoderma. [Conclusion] We explored the mycoparasitism mechanism of Cladosporium at the genome level, providing reference information for further studying the mycoparasitism mechanism and mining the secondary metabolites, which is of great significance for the subsequent development and utilization of Cladosporium.