Assessment of Listeria monocytogenes contamination in retail fresh pork in the post-COVID-19 era

doi:10.13344/j.microbiol.china.211181

Home > Archive>Volume 49, Issue 8, 2022 >3220-3231. DOI:10.13344/j.microbiol.china.211181

Assessment of Listeria monocytogenes contamination in retail fresh pork in the post-COVID-19 era
DOI:
                        10.13344/j.microbiol.china.211181
                    
CSTR:
                        32113.14.j.MC.211181
                    
Author:
                        ZHANG YuanyuanZHANG Yuanyuan
Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, National Center of Meat Quality and Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China
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ZHAO ZiyuZHAO Ziyu
Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, National Center of Meat Quality and Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China
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ZHANG YanningZHANG Yanning
Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, National Center of Meat Quality and Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China
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WEI YeyuWEI Yeyu
Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, National Center of Meat Quality and Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China
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BAI LiBAI Li
China National Center for Food Safety Risk Assessment, Beijing 100021, China
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WANG YeruWANG Yeru
China National Center for Food Safety Risk Assessment, Beijing 100021, China
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YE KepingYE Keping
Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, National Center of Meat Quality and Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China
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[Background] The outbreak of corona virus disease 2019 (COVID-19) at the end of 2019 posed a major challenge to food safety. [Objective] To assess the contamination of Listeria monocytogenes in commercial fresh pork in Nanjing in the post-COVID-19 era. [Methods] We collected the fresh pork samples from different locations and in different packages and quarters during 2020-2021, and then analyzed the contamination rate and level of L. monocytogenes and the epidemiological characteristics of the isolates. [Results] The contamination rate of L. monocytogenes in fresh pork was 15.28% (77/504). The contamination rates of pork from open-air market and pork direct-sale stores were higher than that from supermarkets. Among different packaging methods, pre-package and simple package had higher contamination rate than bulk package. Further, the contamination rate varied significantly among different quarters, being the highest (27.78%) in the third quarter. Quantitative results demonstrated that 40.26% of the positive samples had the contamination level exceeding 10 MPN/g (MPN: most probable number). In particular, the contamination level of three samples exceeded 100 MPN/g. The results of serotyping showed that 1/2a-3a (48.05%) and 1/2c-3c (44.16%) were the main serotypes. Moreover, 19.50% of the isolates were multi-antibiotic resistant, and 2 isolates (2.60%) were sensitive to all the test antibiotics. Among the 77 isolates, 68 (88.30%), 46 (59.70%), and 45 (58.40%) were resistant to oxacillin, ampicillin, and cefotaxime, respectively. [Conclusion] In the post-COVID-19 era, the L. monocytogenes contamination of fresh pork varied among different locations, packaging methods, and quarters. A few products had high contamination levels, and the serotype distribution and resistance characteristics were diverse. Therefore, it is essential to strengthen food safety supervision to reduce the occurrence of foodborne diseases.

Key words:fresh pork;Listeria monocytogenes;serotype;antibiotic resistance;post-COVID-19 era;contamination

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ZHANG Yuanyuan, ZHAO Ziyu, ZHANG Yanning, WEI Yeyu, BAI Li, WANG Yeru, YE Keping. Assessment of Listeria monocytogenes contamination in retail fresh pork in the post-COVID-19 era[J]. Microbiology China, 2022, 49(8): 3220-3231

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Received:December 12,2021
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Adopted:January 31,2022
Online: July 28,2022
Published: August 20,2022

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