Mycovirus diversity in Chinese Lentinula edodes core collections

Abstract:

[Background] Viruses in Lentinula edodes lie latent for long periods before the appearance of symptoms. However, once reactivated, they will cause great economic loss. [Objective] To analyze the mycovirus diversity in Chinese L. edodes core collections. [Methods] RT-PCR and RT-qPCR were used to detect 34 types of mycoviruses in 56 core collections of Chinese L. edodes. [Results] The target genes of 14 and 5 types of viruses in most of the positive strains showed bright and faint bands, respectively. The brightness of the bands was positively correlated with virus content. To be specific, the higher the virus content in the positive strain, the brighter the band. Among the 34 viruses, LeFV5 and LeMV1 were detected in all the 56 core collections. The detection rates of 8 viruses in 21 cultivated strains and 6 viruses in 35 wild strains were over 80% and 4 viruses (LeDFV2, LeFV2, LeSV, and LeHV2) were shared by the two. The cultivated strains and wild strains carried 8-21 and 9-23 viruses, respectively, with 16.4 and 16.7 on average. [Conclusion] L. edodes germplasms generally carry a variety of complex mycoviruses. The amplified bands have different brightness in positive strains. L. edodes germplasms with great genetic difference share some viruses with high detection rate, suggesting that these viruses arose early in the evolution of L. edodes.