Optimization of fermentation medium and preparation of effervescent granules of Bacillus velezensis CC0955
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    Abstract:

    [Background] Biocontrol of plant diseases and development of biocontrol products have been the research focuses in plant protection. However, most of the existing biocontrol agents feature a few available dosage forms (wettable powder or aqueous solution), short shelf life, and inconvenient transport and use. [Objective] To optimize the fermentation medium of Bacillus velezensis CC0955 and develop a novel biocontrol agent “effervescent granules”, which is easy to use and preserve. [Methods] Plackett-Burman design, central composite design, and response surface methodology were used to optimize the composition of CC0955 fermentation medium. With the evaluation indicators of pH and bacterial viable count, the composition of CC0955 effervescent granules was optimized by L9(33) orthogonal design, and the physical properties and antifungal effect of the effervescent granules were assessed. [Results] The optimal fermentation medium for CC0955 was composed of peptone 12.00 g, yeast powder 1.00 g, glucose 15.00 g, MgSO4·7H2O 0.40 g, K2HPO4 0.05 g, and distilled water 1 L. After 48 h fermentation with this medium, the inhibition of the culture broth on Rhizoctonia solani reached 89.78%. The optimal composition of effervescent granules is as follows: alkali-to-acid molar ratio of 2.00, silica of 1.50 g, and potassium fulvic acid of 0.03 g. The effervescent granules showed the average disintegration time of 61 s and the pH of the disintegrated solution was 5.44. In addition, the half maximal effective concentration (EC50) against R. solani was 27.65 mg/mL, and the number of viable bacteria was 6.05×108 CFU/g. The survival rate of bacteria was 58.68% after six months of storage at room temperature. Pot experiment showed that the control rate of CC0955 effervescent granule solution on rice sheath blight was 42.35%, and that on tomato gray mold was 57.62%. [Conclusion] The CC0955 effervescent granule is a novel formulation against phytopathogens, which can be used for the control of farmland diseases.

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WU Tianshang, XUE Yarong, LIU Changhong. Optimization of fermentation medium and preparation of effervescent granules of Bacillus velezensis CC0955[J]. Microbiology China, 2022, 49(7): 2599-2611

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History
  • Received:November 05,2021
  • Revised:
  • Adopted:December 31,2021
  • Online: July 06,2022
  • Published: July 20,2022