Differential expression of key genes in pheromone signaling pathway in fruiting body development of Flammulina filiformis

Abstract:

[Background] Fruiting body is the important structure for fungal reproduction and growth and its development is regulated by a variety of signaling pathways. [Objective] In this study, we analyzed the genes in pheromone signaling pathway in Flammulina filiformis on the basis of the transcriptome and genome data to obtain differentially expressed genes, and then determined their expression during mycelia growth and fruiting body development, hoping to provide a reference for studying the fruiting body development of edible fungi. [Methods] We annotated the pheromone signaling pathway of F. filiformis based on genomic data. The key genes involved in the development of F. filiformis fruiting body in this pathway were further identified through transcriptome sequencing. Finally, we verified the key genes by real-time fluorescent quantitative PCR. [Results] The expression of cdc24 and ste12 gene was significantly different in the five samples (primordium, stipe at the elongation stage, pileus at the elongation stage, stipe at the mature stage, and pileus at the mature stage) collected during fruiting body development. PCR results supported the above conclusion. [Conclusion] The key genes cdc24 and ste12 may be involved in the regulation of tissue differentiation during fruiting body development of F. filiformis.

Key words:Flammulina filiformis;MAPK signaling pathway;real-time quantitative PCR;differential expression;fruiting body