Bioinformatics analysis and relative expression of Vvhsp60 gene in Volvariella volvacea under low temperature stress

Abstract:

[Background] Facing temperature stress, organisms produce a large amount of heat shock protein in a short time, which can restore the conformations of damaged proteins and enhance the tolerance of organisms to stress. [Objective] To preliminarily explore the relationship between heat shock protein Vvhsp60 and low temperature tolerance of Volvariella volvacea, and thereby lay a theoretical foundation for further genetic improvement of V. volvacea for low-temperature tolerance. [Methods] The bioinformation of Vvhsp60 was analyzed. The expression of Vvhsp60 gene in low temperature-sensitive strain V23 and low temperature-tolerant strain VH3 under low temperature stress and under heat shock-low temperature treatment was analyzed by real-time fluorescent quantitative PCR (RT-qPCR). [Results] With no signal peptide, Vvhsp60 is not a secretory protein. It exerts biological functions in mitochondria and cytoplasm and is a bidirectional transmembrane protein. Low temperature significantly increased the expression of Vvhsp60 gene in mycelia of both V23 and VH3, and the expression in VH3 was significantly higher than that in V23, which suggests that the high expression of Vvhsp60 gene may help enhance the low-temperature tolerance of VH3. After heat shock treatment, the expression of Vvhsp60 gene in the two strains was significantly higher than that in those without heat shock treatment, indicating that heat shock can induce the expression of Vvhsp60 gene. [Conclusion] Vvhsp60 gene is related to the low-temperature tolerance of V. volvacea, and heat shock can induce the expression of Vvhsp60 gene.

Key words:Volvariella volvacea;low-temperature stress;Vvhsp60 gene;real-time fluorescent quantitative PCR