Research progress in the detection methods of endophytic bacteria

Abstract:

Plant host,together with all associated microorganisms living on or in it,constitute a plant holobiont,and the hologenome is comprised of the genomes of host and microbiota.In recent years,the structure and function of plant microbiota have gradually been elucidated.As a part of plant microbiota,endophytic bacteria colonize inside plant tissues.Therefore,endophytic bacteria have closer and more direct interaction with plants and are less affected by environmental changes than epiphytic or environmental bacteria.This review introduced endophytic bacteria and summarizes the research progress in the development of specific detection methods for endophytic bacteria.Improved and standard analytical methods will greatly facilitate the elucidation of the interaction and symbiosis mechanisms between plants and their endophytic bacteria,leading to new strategies for improving plant nutrition and disease resistance from the perspective of endophytic bacteria.

Key words:endophytic bacteria;endophytic bacterial community;surface sterilization;16S rRNA gene;fluorescence in situ hybridization;next-generation sequencing of 16S rRNA gene amplicons