Optimization of compound amino acid medium for Lentinula edodes and the effect on mycelial growth and physiology

doi:10.13344/j.microbiol.china.210779

Home > Archive>Volume 49, Issue 2, 2022 >545-555. DOI:10.13344/j.microbiol.china.210779

Optimization of compound amino acid medium for Lentinula edodes and the effect on mycelial growth and physiology
DOI:
                        10.13344/j.microbiol.china.210779
                    
CSTR:
                        32113.14.j.MC.210779
                    
Author:
                        CHANG TingtingCHANG Tingting
College of Food Sciences & Technology, Shanghai Ocean University, Shanghai 201306, China;Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences; Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture and Rural Affairs; National Engineering Research Center of Edible Fungi; National R&D Center for Edible Fungi Processing; Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai 201403, China
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ZHAO YanZHAO Yan
College of Food Sciences & Technology, Shanghai Ocean University, Shanghai 201306, China;Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences; Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture and Rural Affairs; National Engineering Research Center of Edible Fungi; National R&D Center for Edible Fungi Processing; Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai 201403, China
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YANG HuanlingYANG Huanling
Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences; Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture and Rural Affairs; National Engineering Research Center of Edible Fungi; National R&D Center for Edible Fungi Processing; Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai 201403, China
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SONG XiaoxiaSONG Xiaoxia
Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences; Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture and Rural Affairs; National Engineering Research Center of Edible Fungi; National R&D Center for Edible Fungi Processing; Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai 201403, China
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DONG QinDONG Qin
Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences; Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture and Rural Affairs; National Engineering Research Center of Edible Fungi; National R&D Center for Edible Fungi Processing; Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai 201403, China
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CHEN MingjieCHEN Mingjie
College of Food Sciences & Technology, Shanghai Ocean University, Shanghai 201306, China;Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences; Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture and Rural Affairs; National Engineering Research Center of Edible Fungi; National R&D Center for Edible Fungi Processing; Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai 201403, China
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Abstract:

[Background] L-amino acid supports the growth and development of Lentinula edodes as a nutrient. It is of great significance for the high-production and high-quality development of L. edodes industry to optimize the growth substrate of L. edodes by adding amino acids. [Objective] Exogenous alanine (Ala), serine (Ser), and asparagine (Asn) were used to improve the medium, and the possible metabolic pathway of the compound amino acids to promote the growth of L. edodes mycelia was explored. [Methods] The three-factor, three-level Box-Behnken design was employed to optimize the concentration of the amino acids, with the evaluation indicator of mycelial growth rate, and a series of physiological indexes of L. edodes mycelia in the optimized medium were determined. [Results] The theoretical optimal concentration of Ala, Asn, and Ser was 174.711 mg/L, 113.826 mg/L, and 5.661 mg/L, respectively, and the formula was verified to effectively promote the growth of L. edodes mycelia. Compared with potato dextrosa agar (PDA), the optimized medium enabled high content of pyruvic acid (PA) and protein, significantly high activity of glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT), and low content of malondialdehyde (MDA) in L. edodes mycelia. [Conclusion] The study provides a theoretical reference for the improvement of L. edodes medium and the mechanism of amino acids promoting mycelial growth.

Key words:Lentinula edodes;amino acid;exogenous addition;response surface methodology

Reference

[1] 边银丙. 食用菌栽培学[M]. 3版. 北京: 高等教育出版社, 2017: 138 Bian YB. Edible Mushroom Cultivation[M]. 3rd ed. Beijing: Higher Education Press, 2017: 138 (in Chinese)

[2] 曹斌, 郭芸芸, 尚团. “波特五力分析”视阙下的日本香菇产业发展特点研究[J]. 中国食用菌, 2019, 38(7): 85-90, 97 Cao B, Guo YY, Shang T. Study on development characteristics of Lentinus edodes industry in Japan based on the porter’s five forces model[J]. Edible Fungi of China, 2019, 38(7): 85-90, 97 (in Chinese)

[3] 曹斌. “十四五”时期推进我国香菇产业高质量发展的前景和实现路径[J]. 食用菌学报, 2020, 27(4): 25-34 Cao B. Prospects and implementation path of promoting high-quality development of Lentinula edodes industry in China during the 14th five-year plan period[J]. Acta Edulis Fungi, 2020, 27(4): 25-34 (in Chinese)

[4] 刘秀明, 陈强, 邬向丽, 黄晨阳. 国内外食用菌增产添加物研究进展[J]. 食用菌学报, 2018, 25(1): 120-125 Liu XM, Chen Q, Wu XL, Huang CY. Usage of mineral and amino acid additives in edible mushroom cultivation[J]. Acta Edulis Fungi, 2018, 25(1): 120-125 (in Chinese)

[5] 王新风, 戴传超, 蒋海龙, 陈珍丽, 石洪娟, 李晶. 硒对栽培平菇产量及营养成分影响的研究[J]. 食品科学, 2005, 26(8): 91-95 Wang XF, Dai CC, Jiang HL, Chen ZL, Shi HJ, Li J. The effect of the Se to the fruiting-body of Pleurotus ostreatus and nutrition composition[J]. Food Science, 2005, 26(8): 91-95 (in Chinese)

[6] 卢娇娇. 铜(Cu²⁺)对糙皮侧耳生长发育的影响研究[D]. 郑州: 河南农业大学硕士学位论文, 2015 Lu JJ. Effects of copper on growth and development of Pleurotus ostreatus[D]. Zhengzhou: Master’s Thesis of Henan Agricultural University, 2015 (in Chinese)

[7] 赵萍. 氨基酸对黑木耳菌丝生长发育的影响[J]. 甘肃农业大学学报, 1997, 32(2): 61-64 Zhao P. Effect of amino acids on growth and development of Auricularia auricula[J]. Journal of Gansu Agricultural University, 1997, 32(2): 61-64 (in Chinese)

[8] Carroll AD, Jr., Schisler LC. Delayed release nutrients for mushroom culture: US3942969[P]. 1976-03-09

[9] Shimokawa T, Nakamura M, Sekiya A, Hiroki A, Tamada M. Grifola froundosa yield-increasing agent, medium for Grifola froundosa fruit-body cultivation using the same, medium or culture medium for proliferating Grifola froundosa mycelium, and Grifola froundosa cultivating method: JP2009201465[P]. 2009-09-10

[10] 宋奇超, 曹凤秋, 巩元勇, 程晓园, 毕昕媛, 刘来华. 高等植物氨基酸吸收与转运及生物学功能的研究进展[J]. 植物营养与肥料学报, 2012, 18(6): 1507-1517 Song QC, Cao FQ, Gong YY, Cheng XY, Bi XY, Liu LH. Current research progresses of amino acids uptake, transport and their biological roles in higher plants[J]. Plant Nutrition and Fertilizer Science, 2012, 18(6): 1507-1517 (in Chinese)

[11] 铃木彰. 食用菌栽培的营养条件与环境调控[J]. 致富之友, 1994(10): 14-15 Ling MZ. Nutritional conditions and environmental regulation of edible fungi cultivation[J]. Friendly Advisor of Wealth-malzing, 1994(10): 14-15 (in Chinese)

[12] 竹玮, 安运平, 魏忠方, 张应香, 文晴. 氨基酸水溶肥对平菇熟料栽培的影响研究[J]. 食用菌, 2019, 41(1): 27-29, 36 Zhu W, An YP, Wei ZF, Zhang YX, Wen Q. Study on the Effect of amino acid water-soluble fertilizer on cultivation of Pleurotus ostreatus clinker[J]. Edible Fungi, 2019, 41(1): 27-29, 36 (in Chinese)

[13] 杨焕玲, 查磊, 赵旭, 赵妍, 王倩, 余昌霞, 陈明杰. 培养基中添加海藻糖对大球盖菇、斑玉蕈菌丝生长的影响[J]. 微生物学通报, 2019, 46(5): 1108-1114 Yang HL, Zha L, Zhao X, Zhao Y, Wang Q, Yu CX, Chen MJ. Effect of adding trehalose to culture medium on the growth of Stropharia rugosoannulata and Hypsizigus marmoreus[J]. Microbiology China, 2019, 46(5): 1108-1114 (in Chinese)

[14] 吴玉群, 史振声, 李荣华, 陈凤玉, 王超男, 王志斌. 植物氨基酸液肥对甜玉米产量及生理指标的影响[J]. 玉米科学, 2006, 14(5): 130-133 Wu YQ, Shi ZS, Li RH, Chen FY, Wang CN, Wang ZB. Effect of plant amino acid liquid fertilizer on the physiological index and yield of sweet corn[J]. Journal of Maize Sciences, 2006, 14(5): 130-133 (in Chinese)

[15] 于会丽, 林治安, 李燕婷, 袁亮, 赵秉强. 喷施小分子有机物对小油菜生长发育和养分吸收的影响[J]. 植物营养与肥料学报, 2014, 20(6): 1560-1568 Yu HL, Lin ZA, Li YT, Yuan L, Zhao BQ. Effects of spraying low molecular organic compounds on growth and nutrients uptake of rape (Brassica Chinensis L.)[J]. Journal of Plant Nutrition and Fertilizer, 2014, 20(6): 1560-1568 (in Chinese)

[16] Delmas J, Mamoun M. Study of some growth and fruiting factors of Pleurotus cornucopiae[J]. Comptes Rendus Des Seances De Lacademie, 1980, 66(3): 294-301

[17] 张政. 氨基酸态氮对黄瓜的营养效应[D]. 重庆: 西南农业大学硕士学位论文, 2005 Zhang Z. Effects of amino acid nitrogen on cucumber growth[D]. Chongqing: Master’s Thesis of Southwest Agricultural University, 2005 (in Chinese)

[18] 张君颜. 灌溉方式对寒地直播稻氮代谢及产量的影响[D]. 哈尔滨: 东北农业大学硕士学位论文, 2019 Zhang JY. Effect of irrigation on nitrogen metabolism and yield of sown rice in cold-region[D]. Harbin: Master’s Thesis of Northeast Agricultural University, 2019 (in Chinese)

[19] 王镜岩, 朱圣庚, 徐长法. 生物化学[M]. 3版. 北京: 高等教育出版社, 2002: 123 Wang JY, Zhu SG, Xu CF. Biochemistry[M]. 3rd ed. Beijing: Higher Education Press, 2002: 123 (in Chinese)

[20] El-Samad HMA, Shaddad MAK, Barakat N. Improvement of plants salt tolerance by exogenous application of amino acids[J]. Journal of Medicinal Plant Research, 2011, 5(24): 5692-5699

[21] 施燕华, 黄玉婷, 束方智, 陆叶, 刘大林. 外源谷氨酸对铝胁迫下多花黑麦草幼苗生长的缓解作用[J]. 草地学报, 2020, 28(6): 1605-1614 Shi YH, Huang YT, Shu FZ, Lu Y, Liu DL. Alleviation of exogenous glutamic acid on seedling growth of Italian ryegrass under aluminum stress[J]. Acta Agrestia Sinica, 2020, 28(6): 1605-1614 (in Chinese)

[22] Teh CY, Ho CL, Shaharuddin NA, Lai KS, Mahmood M. Proteome of rice roots treated with exogenous proline[J]. 3 Biotech, 2019, 9(3): 1-8

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CHANG Tingting, ZHAO Yan, YANG Huanling, SONG Xiaoxia, DONG Qin, CHEN Mingjie. Optimization of compound amino acid medium for Lentinula edodes and the effect on mycelial growth and physiology[J]. Microbiology China, 2022, 49(2): 545-555

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Received:August 24,2021
Revised:October 03,2021
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Online: February 21,2022
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