Effects of multiple strains fermentation and enzymatic hydrolysis on nutrient composition and quality of soybean hulls
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    Abstract:

    [Background] Soybean hulls as a crude feed have high digestibility, but their fiber content is high, and contains a variety of anti-nutritional factors such as urease and antigen protein, which limits its application in monogastric animal feed. [Objective] The aims of this study to reduce the content of fiber and anti-nutritional factors in soybean hulls by combination of bacteria fermentation and enzymatic hydrolysis. The packaging treatment of soybean hulls by using this method was conducted in the laboratory to evaluate the number of molds and the nutritional value and quality of soybean hulls for the foundation of future industrial application of soybean hulls. [Methods] The experiment was divided into four groups:control group (soybean hulls without fermentation and enzymatic hydrolysis group), strain fermentation group (Lactobacillus, Bacillus, and Saccharomyces mixture fermentation), enzymatic hydrolysis group (cellulase and xylanase enzymatic hydrolysis), and enzymatic hydrolysis + strain fermentation group (Lactobacillus, Bacillus and Saccharomyces compound fermentation + cellulase and xylanase enzymatic hydrolysis). The optimal time for packaging treatment was selected. [Results] Compared with the control group, the pH value, reducing sugar content, urease activity, globulin, and β-conglycinin of the fermentation group were decreased (P<0.05). The reducing sugar, crude protein, and true protein content in the enzymatic hydrolysis group was increased (P<0.05), while the neutral detergent fiber and acid detergent fiber were decreased (P<0.05). The pH value, the contents of neutral detergent fiber, and acid detergent fiber were decreased (P<0.05), while the reducing sugar content, crude protein, and true protein contents were increased (P<0.05) in the bacteria fermentation and enzymatic hydrolysis group. Compared with the fermentation group, lactic acid concentration, and viable bacteria number were higher, while pH value, urease activity, globulin, and β-conglycinin were decreased in the bacteria fermentation and enzymatic hydrolysis group (P<0.05). Compared with the enzyme hydrolysis group, the reducing sugar concentration, the neutral detergent fiber, acid detergent fiber, and hemicellulose in the bacteria fermentation and enzymatic hydrolysis group were decreased (P<0.05). In the bag experiment, the pH value of the enzyme fermentation group was 4.87 at the optimal fermentation time of 5 d, which was lower than that of the other groups (P<0.05). Compared with the fermentation group, the reducing sugar content, crude protein, and true protein in the bacteria fermentation and enzymatic hydrolysis group were increased (P<0.05), while the urease activity, globulin, β-conglycinin, neutral detergent fiber, acid detergent fiber and, hemicellulose in the bacteria fermentation and enzymatic hydrolysis group were decreased (P<0.05). Compared with the enzymatic hydrolysis group, the reducing sugar content, neutral detergent fiber, acid detergent fiber and, hemicellulose in the bacteria fermentation and enzymatic hydrolysis group were decreased (P<0.05). The content of mold in the bacterial enzyme fermentation group was only 0.89 lg(CFU/mL), which was lower than that in the other groups (P<0.05). [Conclusion] The combination of bacteria and enzymes can increase the crude protein and true protein content, reduce the content of fiber and anti-nutritional factors, inhibit the production of mold in soybean hulls, and finally improve the nutritional value and quality of soybean hulls.

    Reference
    [1] Corredor DY, Sun XS, Salazar JM, Hohn KL, Wang D. Enzymatic hydrolysis of soybean hulls using dilute acid and modified steam-explosion pretreatments[J]. Journal of Biobased Materials and Bioenergy, 2008, 2(1):43-50
    [2] Tabibloghmany FS, Mazaheri Tehrani M, Koocheki A. Optimization of the extrusion process through response surface methodology for improvement in functional and nutritional properties of soybean hull[J]. Journal of Food Science and Technology, 2020, 57(11):4054-4064
    [3] Cho MJ, Unklesbay N, Hsieh FH, Clarke AD. Hydrophobicity of bitter peptides from soy protein hydrolysates[J]. Journal of Agricultural and Food Chemistry, 2004, 52(19):5895-5901
    [4] Drewnowski A, Gomez-Carneros C. Bitter taste, phytonutrients, and the consumer:a review[J]. The American Journal of Clinical Nutrition, 2000, 72(6):1424-1435
    [5] Wang RH, Shaarani SM, Godoy LC, Melikoglu M, Vergara CS, Koutinas A, Webb C. Bioconversion of rapeseed meal for the production of a generic microbial feedstock[J]. Enzyme and Microbial Technology, 2010, 47(3):77-83
    [6] Harith ZT, Charalampopoulos D, Chatzifragkou A. Rapeseed meal hydrolysate as substrate for microbial astaxanthin production[J]. Biochemical Engineering Journal, 2019, 151:107330
    [7] Christensen P, Glitsø V, Pettersson D, Wischmann B. Fibre degrading enzymes and Lactobacillus plantarum influence liquid feed characteristics and the solubility of fibre components and dry matter in vitro[J]. Livestock Science, 2007, 109(1/2/3):100-103
    [8] 李英英. 菌酶结合发酵改善大豆皮营养价值及品质的研究[D]. 南京:南京农业大学硕士学位论文, 2021 Li YY. Combination of enzymatic hydrolysis and bacteria fermentation to improve the nutritional values and quality of soybean hulls[D]. Nanjing:Master's Thesis of Nanjing Agricultural University, 2021(in Chinese)
    [9] Miller GL. Use of dinitrosalicylic acid reagent for determination of reducing sugar[J]. Analytical Chemistry, 1959, 31(3):426-428
    [10] 叶磊, 杨学敏. 微生物检测技术[M]. 北京:化学工业出版社, 2009 Ye L, Yang XM. Microbial Detection Technology[M]. Beijing:Chemical Industry Press, 2009(in Chinese)
    [11] 张丽英. 饲料分析及饲料质量检测技术[M]. 3版. 北京:中国农业大学出版社, 2007 Zhang LY. Feed Analyses and Quality Test Technology[M]. 3rd ed. Beijing:China Agricultural university Press, 2007. (in Chinese)
    [12] Spagnuolo M, Crecchio C, Pizzigallo MDR, Ruggiero P. Synergistic effects of cellulolytic and pectinolytic enzymes in degrading sugar beet pulp[J]. Bioresource Technology, 1997, 60(3):215-222
    [13] Koo B, Kim JW, Nyachoti CM. Nutrient and energy digestibility, and microbial metabolites in weaned pigs fed diets containing Lactobacillus-fermented wheat[J]. Animal Feed Science and Technology, 2018, 241:27-37
    [14] Gao K, Rehmann L. ABE fermentation from enzymatic hydrolysate of NaOH-pretreated corncobs[J]. Biomass and Bioenergy, 2014, 66:110-115
    [15] Chen J, Stokes MR, Wallace CR. Effects of enzyme-inoculant systems on preservation and nutritive value of haycrop and corn silages[J]. Journal of Dairy Science, 1994, 77(2):501-512
    [16] Chilson JM, Rezamand P, Drewnoski ME, Price W, Hunt CW. Effect of homofermentative lactic acid bacteria and exogenous hydrolytic enzymes on the ensiling characteristics and rumen degradability of alfalfa and corn silages[J]. The Professional Animal Scientist, 2016, 32(5):598-604
    [17] Kuo CH, Huang CY, Shieh CJ, Wang HMD, Tseng CY. Hydrolysis of orange peel with cellulase and pectinase to produce bacterial cellulose using Gluconacetobacter xylinus[J]. Waste and Biomass Valorization, 2019, 10(1):85-93
    [18] Juhász T, Szengyel Z, Réczey K, Siika-Aho M, Viikari L. Characterization of cellulases and hemicellulases produced by Trichoderma reesei on various carbon sources[J]. Process Biochemistry, 2005, 40(11):3519-3525
    [19] 谢凤莲, 常娟, 尹清强, 王平, 吕战旗, 张红英, 杨明凡, 宁长申. 复合益生菌和纤维素酶发酵对艾草营养品质和微观结构的影响[J]. 动物营养学报, 2020, 32(4):1768-1777 Xie FL, Chang J, Yin QQ, Wang P, Lyu ZQ, Zhang HY, Yang MF, Ning CS. Effects of compound probiotics and cellulase fermentation on nutritional quality and microstructure of Artemisia argyi[J]. Chinese Journal of Animal Nutrition, 2020, 32(4):1768-1777(in Chinese)
    [20] Shi CY, He J, Yu J, Yu B, Huang ZQ, Mao XB, Zheng P, Chen DW. Solid state fermentation of rapeseed cake with Aspergillus niger for degrading glucosinolates and upgrading nutritional value[J]. Journal of Animal Science and Biotechnology, 2015, 6(1):13
    [21] Cheng W, Chiu CS, Guu YK, Tsai ST, Liu CH. Expression of recombinant phytase of Bacillus subtilis E20 in Escherichia coli HMS 174 and improving the growth performance of white shrimp, Litopenaeus vannamei, juveniles by using phytase-pretreated soybean meal-containing diet[J]. Aquaculture Nutrition, 2013, 19(2):117-127
    [22] Yamamoto T, Iwashita Y, Matsunari H, Sugita T, Furuita H, Akimoto A, Okamatsu K, Suzuki N. Influence of fermentation conditions for soybean meal in a non-fish meal diet on the growth performance and physiological condition of rainbow trout Oncorhynchus mykiss[J]. Aquaculture, 2010, 309(1/2/3/4):173-180
    [23] Zhou F, Song WX, Shao QJ, Peng X, Xiao JX, Hua Y, Owari BN, Zhang TZ, Ng WK. Partial replacement of fish meal by fermented soybean meal in diets for black sea bream, Acanthopagrus schlegelii, juveniles[J]. Journal of the World Aquaculture Society, 2011, 42(2):184-197
    [24] Shiu YL, Wong SL, Guei WC, Shin YC, Liu CH. Increase in the plant protein ratio in the diet of white shrimp, Litopenaeus vannamei (Boone), using Bacillus subtilis E20-fermented soybean meal as a replacement[J]. Aquaculture Research, 2015, 46(2):382-394
    [25] Shiu YL, Hsieh SL, Guei WC, Tsai YT, Chiu CH, Liu CH. Using Bacillus subtilis E20-fermented soybean meal as replacement for fish meal in the diet of orange-spotted grouper (Epinephelus coioides, Hamilton)[J]. Aquaculture Research, 2015, 46(6):1403-1416
    [26] Chiu ST, Wong SL, Shiu YL, Chiu CH, Guei WC, Liu CH. Using a fermented mixture of soybean meal and earthworm meal to replace fish meal in the diet of white shrimp, Penaeus vannamei (Boone)[J]. Aquaculture Research, 2016, 47(11):3489-3500
    [27] 王旭明, 倪永珍, 李维炯, 陈宗泽, 袁毅. 有效微生物群(EM)对饲料pH值及营养价值的影响[J]. 浙江大学学报:农业与生命科学版, 2002, 28(4):431-434 Wang XM, Ni YZ, Li WJ, Chen ZZ, Yuan Y. Effects of EM on pH value and nutritive value of feed[J]. Journal of Zhejiang Agricultural University:Agric& Life Sci, 2002, 28(4):431-434(in Chinese)
    [28] Barbosa C, Mendes-Faia A, Mendes-Ferreira A. The nitrogen source impacts major volatile compounds released by Saccharomyces cerevisiae during alcoholic fermentation[J]. International Journal of Food Microbiology, 2012, 160(2):87-93
    [29] 肖连冬, 马红霞. 活菌益生高蛋白饲料的生产及应用[J]. 饲料工业, 2007, 28(13):52-54 Xiao LD, Ma HX. Production and application of probiotic high protein feed[J]. Feed Industry, 2007, 28(13):52-54(in Chinese)
    [30] Bi H, Zhao HZ, Lu FX, Zhang C, Bie XM, Lu ZX. Improvement of the nutritional quality and fibrinolytic enzyme activity of soybean meal by fermentation of Bacillus subtilis[J]. Journal of Food Processing and Preservation, 2015, 39(6):1235-1242
    [31] Assohoun MCN, Djeni TN, Koussémon-Camara M, Brou K. Effect of fermentation process on nutritional composition and aflatoxins concentration of Doklu, a fermented maize based food[J]. Food and Nutrition Sciences, 2013, 4(11):1120-1127
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LI Yingying, ZHU Chongmiao, ZHU Pinghua, DING Liren, CAO Xinhua, LI Qi, LI Yan, HANG Suqin. Effects of multiple strains fermentation and enzymatic hydrolysis on nutrient composition and quality of soybean hulls[J]. Microbiology China, 2022, 49(1): 61-71

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History
  • Received:May 26,2021
  • Adopted:June 17,2021
  • Online: December 30,2021
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