Research progress and prospects of Aspergillus oryzae heterologous protein expression system

Abstract:

Aspergillus oryzae as an important industrial microorganism, in heterologous protein expression has been widely used. Limited by the modification and secretion process of the expressed protein, the gene donors currently used in actual production are mainly limited to other fungi, especially filamentous fungi. When exogenous genes were derived from plants, insects and mammals, Aspergillus oryzae produced heterologous protein yield and biological activity is often not satisfactory. This paper reviews the research progress of Aspergillus oryzae as a host for expressing heterologous proteins, including its existing genetic manipulation methods and the application and exploration of heterologous expression. In addition, the application prospects and development directions of Aspergillus oryzae expressing heterologous proteins are prospected.