Research progress on sucrose phosphorylase

Abstract:

Sucrose phosphorylase (SPase), belonging to the glycoside hydrolase family 13, can catalyze the reversible phosphorolysis of sucrose. Thanks to the broad substrate specificity, SPase can transfer the glucosyl to different acceptors to synthesize arbutin, glycosylglycerol, oligosaccharides, polyphenol derivatives, etc. These products are widely used in food, medicine, cosmetics, and other industries. Owing to the development of enzyme catalysis technology and protein engineering, SPase has received more and more attention, with the application extended. This article reviewed the sources, structures and functions, and application fields of SPase, discussed the protein engineering methods for the enzyme and the limitations, and summarized the future research directions.