Colonization characteristics of Pseudomonas poae HT1 in roots and stems of faba bean and its effect on endophytic bacterial diversity

Abstract:

[Background] There are few reports on the colonization of endophytic control bacteria in faba bean and the diversity of endophytic bacteria. [Objective] To investigate the colonization characteristics of Pseudomonas poae HT1 in faba bean and its effect on the diversity of endophytic bacterial community in the rhizome. [Methods] The colonization characteristics of strain HT1 were determined by antibiotic labeling method, and the community diversity of endophytic bacteria in root and stem of strain HT1 was analyzed by high-throughput sequencing technology. [Results] The number of colonization of strain HT1 was root>stem>leaf, which increased first and then decreased. The root, stem and leaf reached the maximum on the 7th day, and the labeled strain could still be detected on the 83rd day. Root irrigation reduced the richness of endophytic bacteria and increased species diversity of endophytic bacteria in roots. The abundances of Firmicutes, Actinobacteria, Bacteroidetes in roots were significantly increased in irrigated root group, while the relative abundances of Proteobacteria in stems were increased but had no significant effect. At the genus level, after root irrigation, the relative abundance of Romboutsia, Mitsuaria, Lactobacillus, Devosia and other genera increased significantly in the root, and the relative abundance of Pseudomonas, Muribaculaceae, Elstera, Sphingomonas in the stem increased significantly. [Conclusion] The strain HT1 could colonize in faba bean plants for more than 83 days, and could affect the composition of microbial community inside the plants and improve the relative abundance of related microorganisms.