Expression of catalase gene (VvCAT1) from Volvariella volvacea in Escherichia coli and its temperature tolerance

Abstract:

[Background] Catalase (CAT) is involved in the growth and development of fungi and protects them from oxidative damage in the event of stress. [Objective] The VvCAT1 gene from Volvariella volvacea was expressed in Escherichia coli Stbl3 and its temperature tolerance was characterized. [Methods] VvCAT1 was cloned to construct a recombinant plasmid pBAR GPE1/VvCAT1 which was then transformed into Stbl3 to yield a recombinant strain expressing catalase. Catalase activity and growth of the recombinant strain (pBAR GPE1/VvCAT1/Stbl3) and the control strain (pBAR GPE1/Stbl3) were measured to verify the temperature tolerance. [Results] The recombinant strain exhibited stronger heat and cold tolerance than the control with increased catalase activity and better growth. [Conclusion] The transgenosis and expression of VvCAT1 significantly improved the heat and cold tolerance of E. coli Stbl3.