Physiological and biochemical characteristics of degenerate mycelium of Flammulina filiformis

Abstract:

[Background] In the process of subculture, Flammulina filiformis strains will degenerate, which affects the yield and quality of F. filiformis strains. [Objective] The physiological and biochemical characteristics of the mycelia of the degenerative strains of F. filiformis were studied in order to select the degenerate strains of F. filiformis. [Methods] This experiment by F. filiformis original strain (H) and degenerated strains (T) as the research object, measuring the different carbon source culture medium on physiological and biochemical characteristics of mycelia and superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activity, and determination of mycelia in the cultivation of laccase (Lac) and the activity of the manganese peroxidase (MnP), recorded the mycelia recovery after scratching the fungi.[Results] The mycelia growth rate of T in each carbon source was lower than that of H, the levels of oidiospore were between 3—4, the activities of SOD and CAT activity were lower than that of H, and the activity of MnP and Lac were the same as H at the 5th day, and lower than H at the 10th, 15th and 20th days. The recovery time of T was longer than H, and the growth thvigor of the restored mycelia was less dense than H. [Conclusion] By exploring the physiological and biochemical characteristics of the mycelium of the original strain and the degenerated strain of F. filiformis, it provides a theoretical basis for judging whether the strain of F. filiformis is the degenerated strain.

Key words:Flammulina filiformis;strain degeneration;mycelium;biological characteristics