De novo sequencing and genomics analysis of a rare marine fungus Stachybotrys longispora FG216

Abstract:

[Background] Stachybotrys longispora FG216 is a rare marine fungus, and its secondary metabolite FGFC1 has fibrinolytic activity. The genome sequence analysis of S. longispora FG216 will enrich and promote the basic research and applied research of synthetic biology of marine microbial functional genes and secondary metabolites. [Objective] To detected the genome sequence of S. longispora FG216, analyze the biological function and homologous similarity relation of the genome, and reveal the related secondary metabolite genes of the fibrinolytic active compound FGFC1. [Methods] Based on Illumina HiSeq high-throughput sequencing platform, S. longispora FG216 strain was de novo sequenced. SSPACE, Augustus and other software were used to perform assembly, encoding gene prediction, gene function annotation, species collinear analysis and prediction of FGFC1 secondary metabolite synthesis gene cluster. [Results] The total sequence length of S. longispora FG216 genome was 45 622 830 bp, and a total of 605 scaffolds were obtained, with a GC content of 51.31%. Annotations predicted 13 329 coding genes and 169 non-coding RNAs. The genome sequencing data submitted to the National Microbiology Science Data Center numbered NMDC60016264, of which 13 053, 8 422, 8 460, 7 714 and 2 847 genes can be matched with the annotation information in NR, KEGG, KOG, GO and CAZy databases respectively. According to comparative genomics analysis, Stachybotrys is conservative, with core genes accounting for 71.44% of the total gene families. S. longispora FG216 and S. chlorohalonata IBT 40285 had the highest similarity. At the same time, 101 secondary metabolite synthesis gene clusters were predicted, among them 18 gene clusters matched the known compounds. Under the antiSMASH predicts that Cluster 57 is a gene cluster encoding the synthesis of the parent nuclear structure of FGFC1 isoindolinone, and the similarity of the gene cluster with S. chlorohalonata IBT 40285 is 40%. [Conclusion] The genome information of the rare marine fungus S. longispora FG216 has been uploaded to the National Microbial Science Data Center for public use, which provides important reference for the study of Stachybotrys species. Cluster 57 was also found to be the partial encoding gene of the parent nucleus of the fibrinolytic active compound FGFC1, a secondary metabolite of S. longispora FG216.

Key words:Stachybotrys longispora;de novo sequencing;gene function annotation;secondary metabolism;comparative genomics