Research progress in the application of bacteriophage lysin

doi:10.13344/j.microbiol.china.201085

Home > Archive>Volume 48, Issue 9, 2021 >3330-3340. DOI:10.13344/j.microbiol.china.201085

Research progress in the application of bacteriophage lysin
DOI:
                        10.13344/j.microbiol.china.201085
                    
CSTR:
                        32113.14.j.MC.201085
                    
Author:
                        ZHOU BiaoZHOU Biao
College of Life Sciences, Fujian Normal University, Fuzhou, Fujian 350117, China;Fujian Normal University, Biomedical Research Center of South, Fuzhou, Fujian 350117, China
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ZHEN XiangkaiZHEN Xiangkai
College of Life Sciences, Fujian Normal University, Fuzhou, Fujian 350117, China;Fujian Normal University, Biomedical Research Center of South, Fuzhou, Fujian 350117, China
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OUYANG SongyingOUYANG Songying
College of Life Sciences, Fujian Normal University, Fuzhou, Fujian 350117, China;Fujian Normal University, Biomedical Research Center of South, Fuzhou, Fujian 350117, China
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Abstract:

More recently, the frequency of multi-drug-resistant strains has sped up with the misuse of antibiotics. Since the number of deaths caused by bacterial infection is increasing year by year and human health is facing a substantial challenge, it is urgent to develop novel antibacterial drugs. Bacteriophage lysin is a type of protein which synthesized by double-stranded DNA bacteriophage at the late stage of genome replication. It can hydrolyze the peptidoglycan in the bacterial cell wall to release the progeny bacteriophage and kill the bacteria. Bacteriophage lysin is derived from bacteriophages and has a unique advantage of evolutionary selection. It is not only efficient and fast quickly kill multi-drug resistant bacteria, and it is not easy to cause bacteria to develop new drug resistance. Because of its highly efficient bactericidal capacity and high host specialization, bacteriophage lysin has become one candidate for a new generation of antibacterial agents. A review on the structure and mode of action of lysins and their application were presented.

Key words:bacteriophage;lysin;antibacterial agent;application

Reference

[1] Loessner MJ. Bacteriophage endolysins:current state of research and applications[J]. Current Opinion in Microbiology, 2005, 8(4):480-487

[2] Gutiérrez D, Fernández L, Rodríguez A, García P. Are phage lytic proteins the secret weapon to kill Staphylococcus aureus?[J]. mBio, 2018, 9(1):e01923-e01917

[3] Roach DR, Donovan DM. Antimicrobial bacteriophage-derived proteins and therapeutic applications[J]. Bacteriophage, 2015, 5(3):e1062590

[4] Broendum SS, Buckle AM, McGowan S. Catalytic diversity and cell wall binding repeats in the phage-encoded endolysins[J]. Molecular Microbiology, 2018, 110(6):879-896

[5] Reuter M, Kruger DH. Approaches to optimize therapeutic bacteriophage and bacteriophage-derived products to combat bacterial infections[J]. Virus Genes, 2020, 56(2):136-149

[6] Hong S, Son B, Ryu S, Ha NC. Crystal structure of LysB4, an endolysin from Bacillus cereus-targeting bacteriophage B4[J]. Molecules and Cells, 2019, 42(1):79-86

[7] Briers Y, Schmelcher M, Loessner MJ, Hendrix J, Engelborghs Y, Volckaert G, Lavigne R. The high-affinity peptidoglycan binding domain of Pseudomonas phage endolysin KZ144[J]. Biochemical and Biophysical Research Communications, 2009, 383(2):187-191

[8] Larpin Y, Oechslin F, Moreillon P, Resch G, Entenza JM, Mancini S. In vitro characterization of PlyE146, a novel phage lysin that targets Gram-negative bacteria[J]. PLoS One, 2018, 13(2):e0192507

[9] Briers Y, Volckaert G, Cornelissen A, Lagaert S, Michiels CW, Hertveldt K, Lavigne R. Muralytic activity and modular structure of the endolysins of Pseudomonas aeruginosa bacteriophages φKZ and EL[J]. Molecular Microbiology, 2007, 65(5):1334-1344

[10] Schmelcher M, Shabarova T, Eugster MR, Eichenseher F, Tchang VS, Banz M, Loessner MJ. Rapid multiplex detection and differentiation of Listeria cells by use of fluorescent phage endolysin cell wall binding domains[J]. Applied and Environmental Microbiology, 2010, 76(17):5745-5756

[11] Jacob F, Fuerst CR. The mechanism of lysis by phage studied with defective lysogenic bacteria[J]. Journal of General Microbiology, 1958, 18(2):518-526

[12] Freimer EH, Krause RM, McCarty M. Studies of L forms and protoplasts of group A streptococci. I. isolation, growth, and bacteriologic characteristics[J]. Journal of Experimental Medicine, 1959, 110(6):853-874

[13] Letrado P, Corsini B, Díez-Martínez R, Bustamante N, Yuste JE, García P. Bactericidal synergism between antibiotics and phage endolysin Cpl-711 to kill multidrug-resistant pneumococcus[J]. Future Microbiology, 2018, 13:1215-1223

[14] Grandgirard D, Loeffler JM, Fischetti VA, Leib SL. Phage lytic enzyme Cpl-1 for antibacterial therapy in experimental pneumococcal meningitis[J]. The Journal of Infectious Diseases, 2008, 197(11):1519-1522

[15] Hermoso JA, Monterroso B, Albert A, Galán B, Ahrazem O, Garcı́a P, Martı́nez-Ripoll M, Garcı́a JL, Menéndez M. Structural basis for selective recognition of pneumococcal cell wall by modular endolysin from phage Cp-1[J]. Structure, 2003, 11(10):1239-1249

[16] Doehn JM, Fischer K, Reppe K, Gutbier B, Tschernig T, Hocke AC, Fischetti VA, Löffler J, Suttorp N, Hippenstiel S, et al. Delivery of the endolysin Cpl-1 by inhalation rescues mice with fatal pneumococcal pneumonia[J]. The Journal of Antimicrobial Chemotherapy, 2013, 68(9):2111-2117

[17] Monterroso B, Albert A, Martínez-Ripoll M, García P, García JL, Menéndez M, Hermoso JA. Crystallization and preliminary X-ray diffraction studies of the complete modular endolysin from Cp-1, a phage infecting Streptococcus pneumoniae[J]. Acta Crystallographica Section D, Biological Crystallography, 2002, 58(Pt9):1487-1489

[18] Cheng Q, Fischetti VA. Mutagenesis of a bacteriophage lytic enzyme PlyGBS significantly increases its antibacterial activity against group B streptococci[J]. Applied Microbiology and Biotechnology, 2007, 74(6):1284-1291

[19] Patras KA, Wescombe PA, Rösler B, Hale JD, Tagg JR, Doran KS. Streptococcus salivarius K12 limits group B Streptococcus vaginal colonization[J]. Infection and Immunity, 2015, 83(9):3438-3444

[20] Lood R, Raz A, Molina H, Euler CW, Fischetti VA. A highly active and negatively charged Streptococcus pyogenes lysin with a rare D-alanyl-L-alanine endopeptidase activity protects mice against streptococcal bacteremia[J]. Antimicrobial Agents and Chemotherapy, 2014, 58(6):3073-3084

[21] Rashel M, Uchiyama J, Ujihara T, Uehara Y, Kuramoto S, Sugihara S, Yagyu KI, Muraoka A, Sugai M, Hiramatsu K, et al. Efficient elimination of multidrug-resistant Staphylococcus aureus by cloned lysin derived from bacteriophage ϕMR11[J]. The Journal of Infectious Diseases, 2007, 196(8):1237-1247

[22] Gu JM, Feng YG, Feng X, Sun CJ, Lei LC, Ding W, Niu FF, Jiao LY, Yang M, Li Y, et al. Structural and biochemical characterization reveals LysGH15 as an unprecedented "EF-hand-like" calcium-binding phage lysin[J]. PLoS Pathogens, 2014, 10(5):e1004109

[23] Xia FF, Li X, Wang B, Gong PJ, Xiao F, Yang M, Zhang L, Song J, Hu LY, Cheng MJ, et al. Combination therapy of LysGH15 and apigenin as a new strategy for treating pneumonia caused by Staphylococcus aureus[J]. Applied and Environmental Microbiology, 2016, 82(1):87-94

[24] Zhang L, Li D, Li XW, Hu LY, Cheng MJ, Xia FF, Gong PJ, Wang B, Ge JL, Zhang H, et al. LysGH15 kills Staphylococcus aureus without being affected by the humoral immune response or inducing inflammation[J]. Scientific Reports, 2016, 6:29344

[25] Barbosa-Ribeiro M, De-Jesus-soares A, Zaia AA, Ferraz CCR, Almeida JFA, Gomes BPFA. Antimicrobial susceptibility and characterization of virulence genes of Enterococcus faecalis isolates from teeth with failure of the endodontic treatment[J]. Journal of Endodontics, 2016, 42(7):1022-1028

[26] Daniel DS, Lee SM, Gan HM, Dykes GA, Rahman S. Genetic diversity of Enterococcus faecalis isolated from environmental, animal and clinical sources in Malaysia[J]. Journal of Infection and Public Health, 2017, 10(5):617-623

[27] Zhang WH, Mi ZQ, Yin XY, Fan H, An XP, Zhang ZY, Chen JK, Tong YG. Characterization of Enterococcus faecalis phage IME-EF₁ and its endolysin[J]. PLoS One, 2013, 8(11):e80435

[28] Zhou B, Zhen XK, Zhou H, Zhao FY, Fan CP, Perčulija V, Tong YG, Mi ZQ, Ouyang SY. Structural and functional insights into a novel two-component endolysin encoded by a single gene in Enterococcus faecalis phage[J]. PLoS Pathogens, 2020, 16(3):e1008394

[29] Proença D, Velours C, Leandro C, Garcia M, Pimentel M, São-José C. A two-component, multimeric endolysin encoded by a single gene[J]. Molecular Microbiology, 2015, 95(5):739-753

[30] Xu XL, Zhang DD, Zhou B, Zhen XK, Ouyang SY. Structural and biochemical analyses of the tetrameric cell binding domain of Lys170 from enterococcal phage F170/08[J]. European Biophysics Journal, 2021, 50(5):721-729

[31] Shen Y, Köller T, Kreikemeyer B, Nelson DC. Rapid degradation of Streptococcus pyogenes biofilms by PlyC, a bacteriophage-encoded endolysin[J]. Journal of Antimicrobial Chemotherapy, 2013, 68(8):1818-1824

[32] Hoopes JT, Stark CJ, Kim HA, Sussman DJ, Donovan DM, Nelson DC. Use of a bacteriophage lysin, PlyC, as an enzyme disinfectant against Streptococcus equi[J]. Applied and Environmental Microbiology, 2009, 75(5):1388-1394

[33] Nelson D, Loomis L, Fischetti VA. Prevention and elimination of upper respiratory colonization of mice by group A streptococci by using a bacteriophage lytic enzyme[J]. Proceedings of the National Academy of Sciences of the United States of America, 2001, 98(7):4107-4112

[34] McGowan S, Buckle AM, Mitchell MS, Hoopes JT, Gallagher DT, Heselpoth RD, Shen Y, Reboul CF, Law RHP, Fischetti VA, et al. X-ray crystal structure of the streptococcal specific phage lysin PlyC[J]. Proceedings of the National Academy of Sciences of the United States of America, 2012, 109(31):12752-12757

[35] Bai J, Kim YT, Ryu S, Lee JH. Biocontrol and rapid detection of food-borne pathogens using bacteriophages and endolysins[J]. Frontiers in Microbiology, 2016, 7:474

[36] Cho JH, Kwon JG, O'Sullivan DJ, Ryu S, Lee JH. Development of an endolysin enzyme and its cell wall-binding domain protein and their applications for biocontrol and rapid detection of Clostridium perfringens in food[J]. Food Chemistry, 2021, 345:128562

[37] Jamal M, Bukhari SMAUS, Andleeb S, Ali M, Raza S, Nawaz MA, Hussain T, Rahman SU, Shah SSA. Bacteriophages:an overview of the control strategies against multiple bacterial infections in different fields[J]. Journal of Basic Microbiology, 2019, 59(2):123-133

[38] Schmelcher M, Waldherr F, Loessner MJ. Listeria bacteriophage peptidoglycan hydrolases feature high thermoresistance and reveal increased activity after divalent metal cation substitution[J]. Applied Microbiology and Biotechnology, 2012, 93(2):633-643

[39] Kretzer J, Schmelcher M, Loessner M. Ultrasensitive and fast diagnostics of viable Listeria cells by CBD magnetic separation combined with A511:detection[J]. Viruses, 2018, 10(11):626

[40] Kretzer JW, Lehmann R, Schmelcher M, Banz M, Kim KP, Korn C, Loessner MJ. Use of high-affinity cell wall-binding domains of bacteriophage endolysins for immobilization and separation of bacterial cells[J]. Applied and Environmental Microbiology, 2007, 73(6):1992-2000

[41] Yi ZJ, Wang SH, Meng XY, Wu AQ, Li Q, Song YJ, Zhao RL, Qiao JJ. Lysin cell-binding domain-functionalized magnetic beads for detection of Staphylococcus aureus via inhibition of fluorescence of Amplex Red/hydrogen peroxide assay by intracellular catalase[J]. Analytical and Bioanalytical Chemistry, 2019, 411(27):7177-7185

[42] Marin Viegas VS, Ocampo CG, Petruccelli S. Vacuolar deposition of recombinant proteins in plant vegetative organs as a strategy to increase yields[J]. Bioengineered, 2017, 8(3):203-211

[43] Islam MR, Son N, Lee J, Lee DW, Sohn EJ, Hwang I. Production of bacteriophage-encoded endolysin, LysP11, in Nicotiana benthamiana and its activity as a potent antimicrobial agent against Erysipelothrix rhusiopathiae[J]. Plant Cell Reports, 2019, 38(12):1485-1499

[44] Matamp N, Bhat S. Phage endolysins as potential antimicrobials against multidrug resistant Vibrio alginolyticus and Vibrio parahaemolyticus:current status of research and challenges ahead[J]. Microorganisms, 2019, 7(3):84

[45] Srinivasan R, Chaitanyakumar A, Subramanian P, Mageswari A, Gomathi A, Aswini V, Sankar AM, Ramya M, Gothandam KM. Recombinant engineered phage-derived enzybiotic in Pichia pastoris X-33 as whole cell biocatalyst for effective biocontrol of Vibrio parahaemolyticus in aquaculture[J]. International Journal of Biological Macromolecules, 2020, 154:1576-1585

[46] Villa TG, Sieiro C. Phage therapy, lysin therapy, and antibiotics:a trio due to come[J]. Antibiotics, 2020, 9(9):604

[47] Oechslin F, Daraspe J, Giddey M, Moreillon P, Resch G. In vitro characterization of PlySK1249, a novel phage lysin, and assessment of its antibacterial activity in a mouse model of Streptococcus agalactiae bacteremia[J]. Antimicrobial Agents and Chemotherapy, 2013, 57(12):6276-6283

[48] Huang L, Luo DH, Gondil VS, Gong YJ, Jia MH, Yan DZ, He J, Hu SC, Yang H, Wei HP. Construction and characterization of a chimeric lysin Cl_yV with improved bactericidal activity against Streptococcus agalactiae in vitro and in vivo[J]. Applied Microbiology and Biotechnology, 2020, 104(4):1609-1619

[49] Xu JJ, Yang H, Bi YL, Li WY, Wei HP, Li YH. Activity of the chimeric lysin ClyR against common Gram-positive oral microbes and its anticaries efficacy in rat models[J]. Viruses, 2018, 10(7):380

[50] Blázquez B, Fresco-Taboada A, Iglesias-Bexiga M, Menéndez M, García P. PL3 amidase, a tailor-made lysin constructed by domain shuffling with potent killing activity against pneumococci and related species[J]. Frontiers in Microbiology, 2016, 7:1156

[51] Vázquez R, Domenech M, Iglesias-Bexiga M, Menéndez M, García P. Csl2, a novel chimeric bacteriophage lysin to fight infections caused by Streptococcus suis, an emerging zoonotic pathogen[J]. Scientific Reports, 2017, 7:16506

[52] Channabasappa S, Chikkamadaiah R, Durgaiah M, Kumar S, Ramesh K, Sreekanthan A, Sriram B. Efficacy of chimeric ectolysin P128 in drug-resistant Staphylococcus aureus bacteraemia in mice[J]. Journal of Antimicrobial Chemotherapy, 2018, 73(12):3398-3404

[53] Septama AW, Panichayupakaranant P. Synergistic effect of artocarpin on antibacterial activity of some antibiotics against methicillin-resistant Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli[J]. Pharmaceutical Biology, 2016, 54(4):686-691

[54] Rao H, Lai PX, Gao Y. Chemical composition, antibacterial activity, and synergistic effects with conventional antibiotics and nitric oxide production inhibitory activity of essential oil from Geophila repens (L.) I.M. johnst[J]. Molecules, 2017, 22(9):1561

[55] Schuch R, Lee HM, Schneider BC, Sauve KL, Law C, Khan BK, Rotolo JA, Horiuchi Y, Couto DE, Raz A, et al. Combination therapy with lysin CF-301 and antibiotic is superior to antibiotic alone for treating methicillin-resistant Staphylococcus aureus-induced murine bacteremia[J]. The Journal of Infectious Diseases, 2014, 209(9):1469-1478

[56] Indiani C, Sauve K, Raz A, Abdelhady W, Xiong YQ, Cassino C, Bayer AS, Schuch R. The antistaphylococcal lysin, CF-301, activates key host factors in human blood to potentiate methicillin-resistant Staphylococcus aureus bacteriolysis[J]. Antimicrobial Agents and Chemotherapy, 2019, 63(4):e02291-18

[57] Totté JEE, Van Doorn MB, Pasmans SGMA. Successful treatment of chronic Staphylococcus aureus-related dermatoses with the topical endolysin staphefekt SA.100:a report of 3 cases[J]. Case Reports in Dermatology, 2017, 9(2):19-25

[58] Kim NH, Park WB, Cho JE, Choi YJ, Choi SJ, Jun SY, Kang CK, Song KH, Choe PG, Bang JH, et al. Effects of phage endolysin SAL200 combined with antibiotics on Staphylococcus aureus infection[J]. Antimicrobial Agents and Chemotherapy, 2018, 62(10):e00731-18

[59] Gondil VS, Harjai K, Chhibber S. Endolysins as emerging alternative therapeutic agents to counter drug-resistant infections[J]. International Journal of Antimicrobial Agents, 2020, 55(2):105844

[60] Wang YC, Khanal D, Chang RYK, Shang XR, Yang H, Britton WJ, Nelson D, Chan HK. Can bacteriophage endolysins be nebulised for inhalation delivery against Streptococcus pneumoniae?[J]. International Journal of Pharmaceutics, 2020, 591:119982

[61] Gondil VS, Dube T, Panda JJ, Yennamalli RM, Harjai K, Chhibber S. Comprehensive evaluation of chitosan nanoparticle based phage lysin delivery system; a novel approach to counter S. pneumoniae infections[J]. International Journal of Pharmaceutics, 2020, 573:118850

[62] Kaur J, Kour A, Panda JJ, Harjai K, Chhibber S. Exploring endolysin-loaded alginate-chitosan nanoparticles as future remedy for staphylococcal infections[J]. AAPS PharmSciTech, 2020, 21(6):233

[63] Sanz-Gaitero M, Van Raaij MJ. Crystallographic structure determination of bacteriophage endolysins[J]. Current Issues in Molecular Biology, 2021, 40:165-188

[64] Golkar Z, Bagasra O, Pace DG. Bacteriophage therapy:a potential solution for the antibiotic resistance crisis[J]. Journal of Infection in Developing Countries, 2014, 8(2):129-136

[65] Nadeem SF, Gohar UF, Tahir SF, Mukhtar H, Pornpukdeewattana S, Nukthamna P, Moula Ali AM, Bavisetty SCB, Massa S. Antimicrobial resistance:more than 70 years of war between humans and bacteria[J]. Critical Reviews in Microbiology, 2020, 46(5):578-599

[66] Yang H, Yu JP, Wei HP. Engineered bacteriophage lysins as novel anti-infectives[J]. Frontiers in Microbiology, 2014, 5:542

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ZHOU Biao, ZHEN Xiangkai, OUYANG Songying. Research progress in the application of bacteriophage lysin[J]. Microbiology China, 2021, 48(9): 3330-3340

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Received:November 18,2020
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Adopted:April 03,2021
Online: September 08,2021
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