Diversity of soil bacterial communities in the eastern margin of the Taklimakan Desert based on Illumina MiSeq sequencing technology

Abstract:

[Background] The Taklimakan Desert is a special geographical location with less human interference and extreme living environment, and it has uniquely bacteria resources. [Objective] To assess the community structure, diversity and factors influencing of bacteria in the eastern margin of the Taklimakan Desert. [Methods] Analysis the physicochemical properties of sand samples collected from the eastern margin of the Taklimakan Desert, and high-throughput sequencing of environmental 16S rRNA genes based on Illumina MiSeq was used to analyze the diversity and factors influencing of bacteria from the eastern margin of the Taklimakan Desert. [Results] The bacteria of eastern margin of the Taklimakan Desert belonged to definite taxonomic bacteria of 21 phylum 42 classes 304 genera, the dominant bacterial phyla were Bacteroidetes (31.26%), Proteobacteria (29.47%), Actinobacteria (15.71%) and Firmicutes (15.69%). At genus level of taxonomic, the dominant genus was Salinimicrobium (10.06%), followed by Halomonas (7.39%), Bacillus (3.25%), Pontibacter (3.14%), Aliifodinibius (2.76%) and Kocuria (1.76%). TP, SO₄^2- and HCO₃^- were significant effects on the microbial community structure of the eastern margin of the Taklimakan Desert. [Conclusion] This study revealed the community structure and the species diversity of bacteria in the eastern margin of the Taklimakan Desert. This study provides significant theoretical references for the investigation of microbial resource in the Taklimakan Desert.