Advances in heterologous protein expression and secretion of Bacillus subtilis

Abstract:

Bacillus subtilis is an important model strain widely used in basic research and industrial production, which has many advantages such as non-pathogenicity, the strong capability of secreting proteins, clear genetic background, so it is an ideal host for expressing and secreting heterologous proteins. At present, there are many heterologous proteins that have been expressed and secreted in Bacillus subtilis, which includes valuable proteins such as amylase, beta-mannanase and protease. Based on the key steps of the expression and secretion of heterologous protein, this paper summarizes the traditional strategies and the latest technologies for heterologous protein production in Bacillus subtilis. In addition, we analyze the difficulties in the present research and propose new suggestions and strategies for the improvement of the production of heterologous protein.