Research progress on high-throughput rapid detection technology of microorganism

Abstract:

Microorganism is ubiquitous in nature, especially foodborne pathogens that cause foodborne diseases. Traditional methods have been already difficult to fit the demands of disease prevention and supervision in the routine work of regulatory authority, so rapid and sensitive high-throughput detection technique has become a research hot spot. Based on the rapid and accurate recognition of microorganism, bi-functional antibodies, aptamers- and phage-based assays are introduced. In terms of multi-target detection, there are three methods that include multiplex PCR, matrix-assisted laser desorption ionization-time of flight mass spectrometry, and biochip. This paper systematically discussed the detection mechanism, advantages and disadvantages, and research progress of each method, hoping to provide theoretical guarantees and reference for the development of rapid high-throughput method for detecting microorganism in the future.