The diversity of cultivable fungi on corridor murals and controlling of microbial deterioration in Xu Xianxiu's tomb of Northern Qi dynasty, Taiyuan
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    Abstract:

    [Background] The tomb murals preserved at the original sites are generally threatened by the microbial disease, and the long-term controlling of these microorganisms is a long-standing problem in the field of cultural heritage conservation. [Objective] we aimed to explore the cultivable fungal diversity on moldy murals of the tomb corridor surfaces, to isolate the dominant fungal strains and screen the long-acting biocides, and provide a scientific support for the control of the mural's fungal disease of this tomb. [Methods] Mural samples with whitish moldy necrosis were carefully collected by sterile swabs. The surface morphology of samples was analyzed by scanning electron microscope (SEM). The culture-dependent method was employed to isolate fungal strains, and combine with molecular techniques for identification, the community composition of fungi was analyzed thereafter. Combined with the laboratory inhibition zone test and the in-situ biocides test, the size of the inhibition zone of different biocides, the in-situ cultivable microbial concentration after biocides application, and the ATP fluorescence values differences were analyzed in order to find a lasting efficacy of the biocides. [Results] There were a large amount of mycelia with conidia, the culturable fungi in the white mycelium samples affilliated to the six genera of Ascomycota phylum, the dominant cultivated fungus was Parengyodontium album (98.13%). After screening and evaluation in laboratory and in-situ tests, it is clear that the most effective biocide was dichlorophene compounds (0.5% dichlorophene with 75% ethanol), and no repeated outbreaks of microorganisms occurred during the 7-year consecutive monitoring period. [Conclusion] P. album was the dominant cultivable fungi that caused mural moldy at tomb corridor. Dichlorophene compound biocide used in the controlling test of Xu Xianxiu's tomb mural of Northern Qi dynasty has achieved the longest timeliness of maintenance; and it is recommended to combine the emergency protection, environmental regulation and follow-up monitoring in the future, as a result, to achieve long-term prevention and control of the microbial hazards to the tomb murals.

    Reference
    [1] Wang XY. Comparison and significance of murals in tombs around the world[J]. National Arts, 2019(6):93-102(in Chinese)汪小洋. 世界墓室壁画的比较与意义[J]. 民族艺术, 2019(6):93-102
    [2] Sanchez-Moral S, Luque L, Cuezva S, Soler V, Benavente D, Laiz L, Gonzalez JM, Saiz-Jimenez C. Deterioration of building materials in Roman catacombs:the influence of visitors[J]. Science of the Total Environment, 2005, 349(1/3):260-276
    [3] Vasanthakumar A, DeAraujo A, Mazurek J, Schilling M, Mitchell R. Microbiological survey for analysis of the brown spots on the walls of the tomb of King Tutankhamun[J]. International Biodeterioration & Biodegradation, 2013, 79:56-63
    [4] Diaz-Herraiz M, Jurado V, Cuezva S, Laiz L, Pallecchi P, Tiano P, Sanchez-Moral S, Saiz-Jimenez C. The actinobacterial colonization of Etruscan paintings[J]. Scientific Reports, 2014, 3:1440
    [5] Capodicasa S, Fedi S, Porcelli AM, Zannoni D. The microbial community dwelling on a biodeteriorated 16th century painting[J]. International Biodeterioration & Biodegradation, 2010, 64(8):727-733
    [6] Mazzoni M, Alisi C, Tasso F, Cecchini A, Marconi P, Sprocati AR. Laponite micro-packs for the selective cleaning of multiple coherent deposits on wall painting:the case study of Casina Farnese on the Palatine Hill (Rome-Italy)[J]. International Biodeterioration & Biodegradation, 2014, 94:1-11
    [7] Leplat J, Francois A, Bousta F. White fungal covering on the wall paintings of the Saint-Savin-sur-Gartempe Abbey church crypt:a case study[J]. International Biodeterioration & Biodegradation, 2017, 122:29-37
    [8] Cennamo P, Montuori N, Trojsi G, Fatigati G, Moretti A. Biofilms in churches built in grottoes[J]. Science of the Total Environment, 2016, 543:727-738
    [9] Bastian F, Jurado V, Nováková A, Alabouvette C, Saiz-Jimenez C. The microbiology of Lascaux Cave[J]. Microbiology, 2010, 156(3):644-652
    [10] An KD, Kiyuna T, Kigawa R, Sano C, Miura S, Sugiyama J. The identity of Penicillium sp. 1, a major contaminant of the stone chambers in the Takamatsuzuka and Kitora Tumuli in Japan, is Penicillium paneum[J]. Antonie van Leeuwenhoek, 2009, 96(4):579-592
    [11] Wu FS, Zhu FQ, Wang WF, Duan YL, Tian T, Feng HY. Review of microbial deterioration and control of Takamatsuzuka Tumulus, Japan[J]. Sciences of Conservation and Archaeology, 2019, 31(3):26-35(in Chinese)武发思, 朱非清, 汪万福, 段育龙, 田恬, 冯虎元. 日本高松冢古坟微生物病害及其防治研究概述[J]. 文物保护与考古科学, 2019, 31(3):26-35
    [12] Ma YT, Zhang H, Du Y, Tian T, Xiang T, Liu XD, Wu FS, An LZ, Wang WF, Gu JD, et al. The community distribution of bacteria and fungi on ancient wall paintings of the Mogao Grottoes[J]. Scientific Reports, 2015, 5:7752
    [13] Ma WX, Wu FS, Tian T, Ma YT, Xiang T, Liu XD, Wang WF, Feng HY. Fungal diversity of brick murals with salt efflorescence and their surroundings in different ancient tombs[J]. Microbiology China, 2018, 45(10):2091-2104(in Chinese)马文霞, 武发思, 田恬, 马燕天, 向婷, 刘贤德, 汪万福, 冯虎元. 墓室酥碱砖壁画及其环境的真菌多样性分析[J]. 微生物学通报, 2018, 45(10):2091-2104
    [14] Ma WX, Wu FS, Tian T, He DP, Zhang Q, Gu JD, Duan YL, Ma DX, Wang WF, Feng HY. Fungal diversity and its contribution to the biodeterioration of mural paintings in two 1700-year-old tombs of China[J]. International Biodeterioration & Biodegradation, 2020, 152:104972
    [15] Guglielminetti M, De Giuli Morghen C, Radaelli A, Bistoni F, Carruba G, Spera G, Caretta G. Mycological and ultrastructural studies to evaluate biodeterioration of mural paintings. Detection of fungi and mites in frescos of the monastery of St Damian in Assisi[J]. International Biodeterioration & Biodegradation, 1994, 33(3):269-283
    [16] Bastian F, Alabouvette C, Jurado V, Saiz-Jimenez C. Impact of biocide treatments on the bacterial communities of the Lascaux Cave[J]. Naturwissenschaften, 2009, 96(7):863-868
    [17] Martin-Sanchez PM, Nováková A, Bastian F, Alabouvette C, Saiz-Jimenez C. Use of biocides for the control of fungal outbreaks in subterranean environments:the case of the Lascaux Cave in France[J]. Environmental Science & Technology, 2012, 46(7):3762-3770
    [18] Alonso L, Pommier T, Kaufmann B, Dubost A, Chapulliot D, Doré J, Douady CJ, Moënne-Loccoz Y. Anthropization level of Lascaux Cave microbiome shown by regional-scale comparisons of pristine and anthropized caves[J]. Molecular Ecology, 2019, 28(14):3383-3394
    [19] Sterflinger K. Fungi:their role in deterioration of cultural heritage[J]. Fungal Biology Reviews, 2010, 24(1/2):47-55
    [20] Kakakhel MA, Wu FS, Gu JD, Feng HY, Shah K, Wang WF. Controlling biodeterioration of cultural heritage objects with biocides:a review[J]. International Biodeterioration & Biodegradation, 2019, 143:104721
    [21] Wu FS, Wu GW, Liu Y, Tian T, Zhang GB, He DP, Xu RH, Zhao LY, Feng HY, Wang WF. Fungal community composition on normal and moldy mural in Xu Xianxiu's tomb of Northern Qi dynasty, Taiyuan[J]. Microbiology China, 2016, 43(3):479-487(in Chinese)武发思, 武光文, 刘岩, 田恬, 张国彬, 贺东鹏, 徐瑞红, 赵林毅, 冯虎元, 汪万福. 太原北齐徐显秀墓壁画真菌群落组成与菌害成因[J]. 微生物学通报, 2016, 43(3):479-487
    [22] Leplat J, François A, Bousta F. Parengyodontium album, a frequently reported fungal species in the cultural heritage environment[J]. Fungal Biology Reviews, 2020, 34(3):126-135
    [23] Tsang CC, Chan JFW, Pong WM, Chen JHK, Ngan AHY, Cheung M, Lai CKC, Tsang DNC, Lau SKP, Woo PCY. Cutaneous hyalohyphomycosis due to Parengyodontium album gen. et comb. nov.[J]. Medical Mycology, 2016, 54(7):699-713
    [24] Tamura K, Dudley J, Nei M, Kumar S. MEGA4:molecular evolutionary genetics analysis (MEGA) software version 4.0[J]. Molecular Biology and Evolution, 2007, 24(8):1596-1599
    [25] Wu FS, Wang WF, He DP, Chen GQ, Ma YT, Zhang GB, Zhang XD, Feng HY. The fungal community composition on mural paintings in Tomb of Wei and Jin dynasty, Jiayuguan[J]. Dunhuang Research, 2013(1):60-66(in Chinese)武发思, 汪万福, 贺东鹏, 陈港泉, 马燕天, 张国彬, 张晓东, 冯虎元. 嘉峪关魏晋墓腐蚀壁画真菌群落组成分析[J]. 敦煌研究, 2013(1):60-66
    [26] Karpovich-Tate N, Rebrikova NL. Microbial communities on damaged frescoes and building materials in the Cathedral of the nativity of the virgin in the Pafnutii-Borovskii monastery, Russia[J]. International Biodeterioration, 1991, 27(3):281-296
    [27] Šimonovičová A, Gódyová M, Kunert J. Engyodontium album, a new species of microscopic fungi for Slovakia and its keratinolytic activity[J]. Biologia, Bratislava, 2004, 59(1):17-18
    [28] Griffin PS, Indictor N, Koestler RJ. The biodeterioration of stone:a review of deterioration mechanisms, conservation case histories, and treatment[J]. International Biodeterioration, 1991, 28(1/4):187-207
    [29] Maillard JY. Antimicrobial biocides in the healthcare environment:efficacy, usage, policies, and perceived problems[J]. Therapeutics and Clinical Risk Management, 2005, 1(4):307-320
    [30] Zhang BL. Research on the preventive control of mould disease in Dunhuang murals[D]. Lanzhou:Master's Thesis of Lanzhou Jiaotong University, 2012(in Chinese)张昺林. 敦煌壁画霉菌病害的防治研究[D]. 兰州:兰州交通大学硕士学位论文, 2012
    [31] Langsrud S, Sundheim G, Borgmann-Strahsen R. Intrinsic and acquired resistance to quaternary ammonium compounds in food-related Pseudomonas spp.[J]. Journal of Applied Microbiology, 2003, 95(4):874-882
    [32] Cortesia C, Lopez GJ, De Waard JH, Takiff HE. The use of quaternary ammonium disinfectants selects for persisters at high frequency from some species of non-tuberculous mycobacteria and may be associated with outbreaks of soft tissue infections[J]. Journal of Antimicrobial Chemotherapy, 2010, 65(12):2574-2581
    [33] Bastian F, Alabouvette C. Lights and shadows on the conservation of a rock art cave:the case of Lascaux Cave[J]. International Journal of Speleology, 2009, 38(1):55-60
    [34] McBain AJ, Ledder RG, Moore LE, Catrenich CE, Gilbert P. Effects of quaternary-ammonium-based formulations on bacterial community dynamics and antimicrobial susceptibility[J]. Applied and Environmental Microbiology, 2004, 70(6):3449-3456
    [35] Ge QY, Pan XX, Li Q, Pan J, Guo H. Application of ATP-bioluminescence method for the evaluation of biocides used for cultural heritage conservation[J]. Sciences of Conservation and Archaeology, 2014, 26(4):39-46(in Chinese)葛琴雅, 潘晓轩, 李强, 潘皎, 郭宏. ATP生物发光法在文物抑菌剂效力检测中的应用[J]. 文物保护与考古科学, 2014, 26(4):39-46
    [36] Heyrman J, Swings J. 16S rDNA sequence analysis of bacterial isolatesfrom biodeteriorated mural paintings in the Servilia tomb (Necropolis of carmona, Seville, Spain)[J]. Systematic and Applied Microbiology, 2001, 24(3):417-422
    [37] Diaz-Herraiz M, Jurado V, Cuezva S, Laiz L, Pallecchi P, Tiano P, Sanchez-Moral S, Saiz-Jimenez C. Deterioration of an Etruscan tomb by bacteria from the order Rhizobiales[J]. Scientific Reports, 2014, 4:3610
    [38] Li YH, Huang Z, Petropoulos E, Ma Y, Shen Y. Humidity governs the wall-inhabiting fungal community composition in a 1600-year tomb of Emperor Yang[J]. Scientific Reports, 2020, 10:8421
    [39] Li YH, Feng YM, Kong ZY, Hokoi S. Optimization and assessment of the protective shed of the Eastern Wu Tomb[J]. Energies, 2020, 13(7):1652
    [40] Wen SJ. A brief analysis of the environmental control in Xuxianxiu tomb in North Qi, Taiyuan[J]. National Geographic, 2015(9):232-233(in Chinese)温少杰. 浅谈太原北齐徐显秀墓的环境控制问题[J]. 华夏地理, 2015(9):232-233
    [41] Yun YL, Wang J. Scientific analysis of the mural pigments from Xu Xianxiu's tomb of the Northern Qi dynasty[J]. Sciences of Conservation and Archaeology, 2020, 32(4):16-25(in Chinese)员雅丽, 王江. 北齐徐显秀墓壁画颜料的科学分析[J]. 文物保护与考古科学, 2020, 32(4):16-25
    [42] Tian T. Bacterial diversity in air and mural paintings and mechanistic of Naumannella cuiyingiana response to lead pigments from the Xu Xianxiu's Catacomb[D]. Lanzhou:Doctoral Dissertation of Lanzhou University, 2018(in Chinese)田恬. 北齐徐显秀墓葬空气和壁画表面细菌多样性及萃英诺曼菌遇铅色变机制研究[D]. 兰州:兰州大学博士学位论文, 2018
    [43] Tian T, Wu FS, Ma YT, Xiang T, Ma WX, Hu WG, Wu GW, An LZ, Wang WF, Feng HY. Description of Naumannella cuiyingiana sp. nov., isolated from a ca. 1500-year-old mural painting, and emended description of the genus Naumannella[J]. International Journal of Systematic and Evolutionary Microbiology, 2017, 67(8):2609-2614
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WU Fasi, MA Wenxia, HE Dongpeng, Tian Tian, WANG Jiang, LIU Yan, Zhang Qi, WU Guangwen, WANG Wanfu, FENG Huyuan. The diversity of cultivable fungi on corridor murals and controlling of microbial deterioration in Xu Xianxiu's tomb of Northern Qi dynasty, Taiyuan[J]. Microbiology China, 2021, 48(8): 2548-2560

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  • Received:June 14,2020
  • Adopted:January 04,2021
  • Online: July 30,2021
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