Correlations analysis of gut microbiota with blood glucose and lipids in the third trimester pregnancy

Abstract:

[Background] Inappropriate gestational weight gain is associated with disorder of pregnant women’s blood glucose and lipids which are closely related to maternal-fetal metabolic diseases. Gut microbiota coordinates the absorption of nutrients by intestinal cells, which may affect maternal-fetal glucose and lipids level. [Objective] To observe the constitution and diversity of gut microbiota from pregnant women with different gestational weight gain, and explore microbiota which may be related to blood glucose and lipids in the third trimester pregnancy. [Methods] A total of 34 excrement samples were selected from pregnant women with different gestational weight gain. 16S rRNA gene sequencing in the V3?V4 region of gut microbiota was acted by high throughput MiSeq method to analysis the constitution and diversity of gut microbiota. KEGG analysis was used to study the metabolic pathway change of different gestational weight gain. By correlations analysis, we can predict gut microbiota associated with gestational weight gain, maternal body mass index and blood glucose and lipids in the third trimester pregnancy. [Results] There was no significant difference in the diversity and constitution of gut microbiota, but the percentage of differential gut microbiota has changed. Correlation analysis found that Ruminococcaceae_UCG-014 sp. was negatively correlated with maternal body mass index (BMI) in the third trimester and gestational weight gain, Lachnospira spp. negatively correlated with blood glucose in the third trimester, Alitipes spp. and Ruminococcus_torques_group spp. were negatively correlated with maternal triglycerides in the third trimester, Parabacteroides spp. positively correlated with high density lipoprotein in the third trimester while negatively correlated with birthweight. Besides, Mucispirillum spp. was negatively correlated with high density lipoprotein in the third trimester. KEGG analysis found that gut microbiota with average gestational weight gain was significantly higher in 31 metabolic pathways including carbohydrate, lipid and amino acid metabolism than lower and higher gestational weight gain. [Conclusion] Gut microbiota may affect blood glucose and lipids level by affecting the metabolism and transport of nutrients, thereby affecting weight gain during pregnancy.