Variation of arbuscular mycorrhiza fungal communities in the rhizosphere soil of Phoebe bournei (Hemsl.) Yang plantations based on high-throughput sequencing analysis

Abstract:

[Background] Phoebe bournei (Hemsl.) Yang is a precious timber species. Clarifying the characteristics of rhizosphere soil microorganism of P. bournei is helpful for scientific management of P. bournei plantations. [Objective] To understand the changes of arbuscular mycorrhiza community in rhizosphere soil of P. bournei (Hemsl.) Yang plantation with forest age after the afforestation. [Methods] The diversity and composition of arbuscular mycorrhiza fungal communities in the rhizosphere soil of P. bournei plantations at different ages were evaluated by high-throughput Illumina Miseq sequencing. Then, we analyzed response characteristics of soil fugal communities in the rhizosphere soil of P. bournei plantations to changing soil nutrient after afforestation. [Results] In total, 342 555 effective sequences and 253 AMF-OTUs were obtained from the rhizosphere soils of 3 forest ages of P. bournei, belonging to 14 genus, 9 families, 5 orders, 1 class, 1 phylum. In terms of alpha diversities, both Chao1 and Shannon indices of AMF community increased with the plantation age of P. bournei, but there was no significant difference among different plantation ages. There was significant difference of β diversity in arbuscular mycorrhiza fungal community composition among different plantation ages. According to the Bray-Curtis dissimilarity analysis, the similarity of AMF community composition was related to plantation ages. The Spearman correlation analysis between species richness and soil factors indicated that the majority soil factors affecting the abundance of arbuscular mycorrhiza fungal communities were pH, total potassium (K), nitrate nitrogen (NO3–-N) and ammonium nitrogen (NH4+-N). Ammonium was significantly correlated with the abundance of dominant arbuscular mycorrhiza fungal molecular virtual species Glomus-Franke-A1-VTX00076 and Glomus-Franke-A1-VTX00269. [Conclusion] Our findings reveal the relationship between soil microbial diversity and the ecosystem of Phoebe bournei (Hemsl.) Yang plantations and provide reference for understanding the relationships between biodiversity and ecosystem functions.