Detection of pathogenic microorganism by single-cell raman spectroscopy: a review

Abstract:

Single-cell raman spectroscopy (SCRS) is an emerging technology that reveals the intrinsic biochemical profiles of individual cells. SCRS is non-destructive, label-free, and independent of cultivation, thus it is deemed as a rapid and efficient technology with low cost. Based on the principles of raman spectroscopy, herein, we summarize research progress and the latest technical direction of this technology in pathogen identification and drug susceptibility test, and then discuss the application feasibility in a clinical laboratory to provide a new option for the detection, identification and antimicrobial susceptibility testing of pathogenic microorganisms in the future.