Sterilization effect of common disinfectants on vaccine strains of Brucella

Abstract:

[Background] Brucella is an important intracellular bacterium to cause brucellosis in animals and humans. The key measure to prevent and control brucellosis is to choose high effective disinfectant to remove Brucella from the environment. [Objective] To evaluate the sterilization effect of common disinfectants on Brucella. [Methods] Nine common commercial disinfectants with high, medium and low concentrations were selected to study their sterilization effect on Brucella abortus, melitensis and suis. At the same time, according to the effective concentration of disinfectants, we determined the minimum inhibitory concentration (MIC) of each disinfectant. [Results] Except for phenol, the other eight disinfectants, including povidone iodine, decamethonium bromide, benzalkonium bromide, glutaraldehyde, compound quaternary ammonium salt, ethanol and two commercial disinfectants, could completely eliminate Brucella within 5 minutes. The bacterial survival rate of iodate acid preparation at low concentration (0.1%) was 0.000 039%?0.000 095%. For phenol, the bacterial survival rate was 0.000 144%?0.000 183% in 5 minutes and 0.000 138%?0.000 193% in 20 minutes at the recommended concentration of 0.5%. The bacterial survival rate of Brucella suis was 0.000 125% in 5 minutes and decreased to 0.000 038% in 20 minutes at the concentration of 1%. MIC results showed that the MIC of quaternary ammonium disinfectants ranged from 0.015 63% to 0.031 25%, and the MIC results of other disinfectants were basically consistent with the minimum recommended concentration of disinfectants. At the same time, it was found that different species of Brucella had different sensitivity to the same disinfectant. [Conclusion] This study provides reference for disinfectant usage scientifically and reasonably in practice.