Chlamydia trachomatis CT225 interacting with vimentin from HeLa cell

Abstract:

[Background] The unique development cycle of Chlamydia trachomatis is completed in inclusion. About 7%–10% genes encode membrane proteins in the inclusion. Therefore, the membrane proteins in the inclusion may play an important role in the development and pathogenesis of Chlamydia. However, its specific functions still need to be further studied. [Objective] In order to further understand the function of the membrane protein CT225 in the inclusion, the association molecules were screen. [Methods] The fusion protein GST-CT225 was first expressed. Then, the interaction molecules of CT225 were screened from the lysate of HeLa cell by affinity chromatography, and analyzed by mass spectrometry. Finally, the candidate proteins were selected and verified by Co-immunoprecipitation, glutathione S-transferase (GST) pull-down test and subcellular localization. [Results] Vimentin was the protein interacted with CT225 according to the score of mass spectrometry. [Conclusion] CT225 interacts with vimentin in HeLa cells, suggesting that CT225 has the relationship with the cytoskeleton integrity, membrane transport and lipid transport.