Advances in nucleic acid isothermal detection technologies for foodborne viruses

Abstract:

Foodborne viruses have become an important pathogen causing food safety incidents worldwide, which poses a severe challenge to the continuous development of new detection technologies. The application of PCR in the pathogen detection has promoted the comprehensive understanding of foodborne viruses. In recent years, nucleic acid thermostatic detection technologies have been developed rapidly, including loop-mediated isothermal amplification, recombinase polymerase amplification (RPA), nucleic acid sequence-based amplification (NASBA), strand displacement amplification, rolling circle amplification, etc. They showed obvious technical advantages in resisting complex matrix interference, low equipment requirements and on-site real-time detection, which become a hot direction in the foodborne virus detection. Therefore, this paper reviews the principles, applications, advantages and disadvantages of nucleic acid thermostatic detection technologies for foodborne viruses in recent years, and prospects the development direction in future.