Optimization of fermentation process of the third-stage seed fermenter in lincomycin production

Abstract:

[Background] Lincomycin is a lincomamide antibiotic that plays an important role in clinical application, few studies can be found on regulating the parameters of third-stage seed fermenter to optimize the lincomycin fermentation process. [Objective] The fermentation process of lincomycin was optimized in order to improve the fermentation titer and market competitiveness. [Methods] The medium and inoculation amount of the third-stage seed fermenter and the transplanting age of third-stage seeds in lincomycin production were optimized. [Results] The concentrations of glucose, starch, corn pulp, soybean meal and ammonium sulfate in the third-stage seed fermenter’s medium were 64.0, 5.0, 15.0, 14.5 and 3.5 g/L, respectively. The inoculation amount of third-stage seed fermenter and the transplanting age of third-stage seeds were 25% and 60 h, respectively. Under the optimized conditions, the fourth-stage fermenter’s titer of lincomycin was up to 7 883 U/mL, which was 10% higher than that of the before optimization. [Conclusion] Regulating the parameters of third-stage seed fermenter in lincomycin production, the fermentation process was preliminary optimized and the fermentation titer was improved. It provides a new idea for optimization of lincomycin fermentation process.

Key words:Streptomyces lincolnensis, Lincomycin, Fermentation process, Optimization