Quantitative analysis of endophytic archaea and its spatiotemporal variation in verticillium wilt and healthy cotton plants from xinjiang

Abstract:

[Background] At present, verticillium wilt of cotton seriously hinders the stable and high yield of cotton and hinders the development of cotton industry. Endophytic bacteria have great potential in biological control, but the variation of endophytic archaea in verticillium wilt of cotton is rarely reported. [Objective] In order to provide theoretical support for the following studies, the quantitative changes of endophytic archaea in cotton plants with verticillium wilt and healthy plants in different growth periods and different planting areas were studied. [Methods] Using MiSeq high-throughput sequencing and TaqMan probe real-time fluorescence quantitative PCR technology, the endophytic archaea of verticillium wilt and healthy strains in Xinjiang cotton were quantitatively analyzed at different growth stages and in different typical ecological regions. [Results] The community composition of endophytic archaea was similar to that of verticillium wilt and healthy cotton plants at different sampling sites and different growth stages in Xinjiang. At different growth stages, the number of endophytic archaea in verticillium wilt and healthy cotton plants in Xinjiang increased first, then decreased, then tended to be flat, and reached the highest value in bud stage. In different regions, the number of endophytic archaea of verticillium wilt cotton in Xinjiang was the highest in the northern region, followed by the eastern region and the southern region. Health plant is the highest in the southern region, followed by the eastern region, the lowest in the northern region. [Conclusion] The quantity of endophytic archaea in cotton plants with verticillium wilt and healthy plants in Xinjiang was significantly different in different growth periods and different spaces, and the overall variation trend was significant.