Cloning and bioinformatics analysis of pectate lyase PL101 from Phytophthora capsici

Abstract:

[Background] Phytophthora capsici is a worldwide soil borne disease, which has serious impact on pepper production all over the world and brings huge economic losses. Pectate lyase (PL), as a kind of important cell wall degrading enzymes, is an important pathogenic factor of the disease. [Objective] The gene of PL was cloned and its bioinformatics characteristics were analyzed to further elucidate its mechanism. [Methods] According to the whole genome sequence of P. capsici, the full-length cDNA of PL101 gene was amplified with high pathogenic strain SD33 as template, and its physical and chemical properties, transmembrane regions, hydrophobicity, structural domain and other biological characteristics were analyzed. [Results] In addition to the analysis of biological characteristics of PL101, the three-dimensional structure modeling of PL101 was also carried out to obtain protein structure with high reliability, and the possible catalytic sites of PL101 were determined as Asp183, Arg212 and Arg272 amino acids. [Conclusion] The cloning and bioinformatics analysis of PL101 will provide a reference for further elucidating the functional characteristics of PL.