Acclimatization and biodegrading efficiency of the naproxen-degrading microbial community

Abstract:

[Background] Naproxen is a widely used drug which is non-steroidal and anti-inflammatory. It has certainly negative impact on the environment while treating human diseases. What’s more, it maybe even endangers the human living environment. [Objective] The way of degrading naproxen pollutants through microorganisms is inexpensive and effective. [Methods] In this paper, naproxen was used as the sole carbon source to cultivate domesticated and highly effective naproxen-degrading bacteria; The high-throughput sequencing technology was used to analyze the microbial community changes of naproxen-degrading bacteria and to identify the types of naproxen-degrading microbial community; Analysis of degrading pathway of naproxen-degrading microbial community by GC-MS. [Results] Finally, a highly effective naproxen-degrading microbial community was mainly Rhodanobacter, and the optimal growth degradation condition of naproxen-degrading microbial community was determined to be 30 °C, pH 7.0, 150 r/min, 10% inoculation amount, with naproxen degrading rate reached 60.58%, and predicting the degradation pathway of naproxen-degrading microbial community. [Conclusion] Obtaining a high-efficiency naproxen- degrading microbial community, and clarifying the degradation mechanism and degradation pathway. The research not only enriches the types of microbial resources, but also lays the theoretical foundation for microbial engineering applications.

Key words:Naproxen, Microbial community acclimatization, Community structure, Degradation pathway