Research progress of 3-ketosteroid-Δ1-dehydrogenase, a key enzyme for steroid microbial conversion

Abstract:

3-ketosteroid-Δ1-dehydrogenase is one of the key enzymes in microbial steroid catabolism, which catalyzes Δ1-dehydrogenation of A ring of 3-ketosteroid substrates. The enzyme not only plays a critical role in the early step of the degradation of the steroid nucleus, but also introduces the double bond at the C1,2 position of the A ring to significantly improve the physiological activity of the steroidal compound. In this paper, the species distribution in microorganisms and sequence characteristics, biological properties, physiological role, catalytic mechanism and molecular modification of 3-ketosteroid-Δ1-dehydrogenase are reviewed. It provides reference for further study of the 3-ketosteroid- Δ1-dehydrogenase application in the steroid biotransformation field.

Key words:3-ketosteroid-Δ1-dehydrogenase, Δ1-dehydrogenation of steroid, Biological characteristics, Physiological role, Catalytic mechanism