Meso-diaminopimelate dehydrogenase for asymmetric synthesis of unnatural chiral D-amino acids: a review

Abstract:

Asymmetric synthesis of unnatural chiral D-amino acids is a research hotspot, using meso-diaminopimelate dehydrogenase, in the field of biocatalysis. Meso-diaminopimelate dehydrogenase has excellent stereoselectivity, and it was used for enzymatic asymmetric synthesis of optically pure chiral D-amino acids, which is widely applied in medicine, food, cosmetics, fine chemicals field. To efficiently synthesize chiral D-amino acids, the methods of biocatalysis should be further developed. In this review, the research status was introduced about the synthesis of D-amino acids catalyzed by meso-diaminopimelate dehydrogenase from Corynebacterium glutamicum, Symbiobacterium thermophilum, Ureibacillus thermosphaericus. It focuses on research progress of meso-diaminopimelate dehydrogenase in mining of new enzyme, catalytic performance, crystal structure, molecular modification, function and catalytic mechanism, new way of synthesizing D-amino acid, etc. Finally, this paper prospected the future research directions and strategies for meso-diaminopimelate dehydrogenase. Therefore, this review has deepened our understanding of meso-diaminopimelate dehydrogenase, and it also provides guidance and information for challenging biosynthetic tasks.