Recent advances in immobilization of enzymes on porous nanomaterials

Abstract:

Enzyme is a green and natural biocatalyst, with the advantages of high activity under moderate conditions and high specificity. However, the high molecular complexity and intrinsic fragility are the challenges for applying the enzymes in the tough reaction conditions for the industry process. The advanced enzyme immobilization technology has great chance to improve the enzymatic activity and stability, bringing new opportunities for the engineering application of enzymes. Porous nanomaterials with large surface area, high porosity, stable mechanical, chemical resistance and superior cost-effectiveness, are ideal carriers of immobilizing enzyme. In this paper, the recent research progress and applications of nanomaterials such as metal organic framework, covalent organic framework and porous microspheres for enzymatic immobilization are reviewed. We mainly introduced the method of carrier for immobilization of enzymes, summarized the characteristics of each carrier. The challenges and future development of the immobilization technology for enzymes by porous nanomaterials have been finally discussed and concluded.

Key words:Enzyme, immobilization, porous nanomaterials, immobilization methods, research progress