Advances in study on structure-function relationship of thermostable α-amylase and its heterologous expression

Abstract:

Thermostable α-amylase is a group of important industrial enzymes famous for their earliest industrial usage tracing back to thousands of years ago. Thanks to their advantages such as thermal stability, high efficiency on starch liquefaction and the easy-to-stock nature, the thermostable α-amylase is widely used in starch-based sugar industries, food fermentation industries, beer and other brewing industries, and textile printing and dyeing associated industries, respectively. This review covered the advances in the studies on the thermostable α-amylase regarding the producer strains selection and construction, structure-function relationship of the enzyme, the ways for improving α-amylase enzyme activity and the approaches of heterologous expression of the enzyme, respectively. In addition, the status quo of thermostable α-amylase exploration worldwide was depicted in general, and the comparison between domestic achievements with the global leading establishments was made in the specific.