Transcriptional regulation of small RNA EsrE in Escherichia coli

Abstract:

[Background] Small RNA EsrE affects cell growth by regulating the expression of succinic acid dehydrogenase in Escherichia coli, the exploration of its regulatory mechanism is conducive to deepening the understanding of EsrE on cell growth. [Objective] To explore the transcriptional regulation mechanism of small RNA EsrE in E. coli. [Methods] Transcriptional regulators were screened by using a dual plasmid reporting system; the interaction between RpoH and PesrE was verified by electrophoretic mobility shift assay (EMSA); qRT-PCR shows effects of the transcriptional regulators on EsrE. [Results] The results of reporting system demonstrated that RpoH up-regulated PesrE, and FabZ down-regulated PesrE. The results of EMSAs showed that RpoH bound the PesrE fragment directly, however FabZ did not. [Conclusion] RpoH is involved in the regulation of esrE transcription by binding the PesrE promoter directly, while FabZ is involved in the transcriptional regulation of EsrE indirectly by other ways.